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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
BRAF (D594G) Recombinant Protein | BRAF recombinant protein
BRAF (D594G) Protein
Applications
Western Blot
Synonyms
BRAF (D594G); BRAF (D594G) Protein; BRAF1; RAFB1; B-raf; B-raf 1; MGC126806; MGC138284; BRAF recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for BRAF recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for BRAF recombinant protein
Recombinant human BRAF (D594G)(381-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_004333.
BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Protein Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2).
BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Protein Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2).
Product Categories/Family for BRAF recombinant protein
References
1. Weber, C K. et al: Protein Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8.
2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653(1):25-40.
2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653(1):25-40.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69kDa
Protein Family
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Product Notes
The BRAF (Catalog #AAA516609) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BRAF (D594G) can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the BRAF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF (D594G), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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