Thrombin, alpha DFP, Bovine Active Protein
Thrombin, alpha DFP, Bovine
Purity
PurifiedPurified from prothrombin using a modification of the Lundblad procedure (1) as described by Nesheim, et al. (2). Purity is determined by SDS-PAGE analysis.
Synonyms
Thrombin; alpha DFP; Bovine; Bovine active protein
Host
Bovine plasma
Purity/Purification
Purified
Purified from prothrombin using a modification of the Lundblad procedure (1) as described by Nesheim, et al. (2). Purity is determined by SDS-PAGE analysis.
Purified from prothrombin using a modification of the Lundblad procedure (1) as described by Nesheim, et al. (2). Purity is determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 50% glycerol/H2O.
Specific Activity
~3800 NIH u/mg
Extinction Coefficient
E1%1cm, 280nm=19.5 (7)
Mode of Action
Serine protease which cleaves fibrinogen to form fibrin. Also responsible for activation of protein C, platelet activation and feedback activation of the procofactors (Factor V and Factor VIII).
Structure
Two subunits, ~Mr 6000 and 31,000
Percent Carbohydrate
~5%
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Thrombin, alpha DFP, Bovine active protein
Proteolytic Activation of Prothrombin: The serine protease a-thrombin is produced by proteolytic activation of the zymogen, prothrombin. The enzyme complex, prothrombinase, catalyzes the proteolysis of two peptide bonds in prothrombin, which gives rise to an NH2-terminal derived F1.2 region and the heterodimer, a-thrombin.
Thrombin is a two chain enzyme composed of an NH2-terminal "A" chain (Mr 6000) and a COOH-terminal "B" chain (Mr 31,000) which remain covalently associated through a single disulfide bond. Human thrombin is 13 amino acids shorter than the bovine thrombin due to a thrombin cleavage site on the human protein that is not present in the bovine protein.
Thrombin is also utilized for site specific cleavage of fusion proteins expressed in bacteria (9-11). A thrombin sensitive site is incorporated between the recombinant protein of interest and peptides or proteins which facilitate purification and/or expression. The target protein is released from the expressed hybrid by cleavage with thrombin. Thrombin can then be easily removed by affinity chromatography.
Thrombin is a two chain enzyme composed of an NH2-terminal "A" chain (Mr 6000) and a COOH-terminal "B" chain (Mr 31,000) which remain covalently associated through a single disulfide bond. Human thrombin is 13 amino acids shorter than the bovine thrombin due to a thrombin cleavage site on the human protein that is not present in the bovine protein.
Thrombin is also utilized for site specific cleavage of fusion proteins expressed in bacteria (9-11). A thrombin sensitive site is incorporated between the recombinant protein of interest and peptides or proteins which facilitate purification and/or expression. The target protein is released from the expressed hybrid by cleavage with thrombin. Thrombin can then be easily removed by affinity chromatography.
Product Categories/Family for Thrombin, alpha DFP, Bovine active protein
NCBI and Uniprot Product Information
Uniprot Description
Thrombin-like snake venom serine protease that clots fibrinogen (FGA) by releasing fibrinopeptide A. According to PubMed:8585090, only cleaves rabbit fibrinogen, whereas no specificity is described in PubMed:3910643 (tests done on bovine fibrinogen). Also acts as a C3 convertase that independently cleaves human C3 and kick-starts the complement cascade. Also increases urokinase-type plasminogen activator (PLAU) and plasminogen activator inhibitor (SERPINE1) in cultured bovine pulmonary artery endothelial cells. Dose-dependently inhibits collagen-induced platelet aggregation.
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Product Notes
The Thrombin, alpha DFP, Bovine (Catalog #AAA634886) is an Active Protein produced from Bovine plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thrombin, alpha DFP, Bovine, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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