Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit | MCP-1 elisa kit

Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit

Cat. Num. AAA734688

• Gene Names: CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Reactivity: Canine
• Synonyms: Monocyte chemotactic protein 1/monocyte chemotactic and activating factor; Canine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit; MCP-1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This MCP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MCP-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP-1 elisa kit

Principle of the assay: MCP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-1 antibody and an MCP-1-HRP conjugate. The assay sample and buffer are incubated together with MCP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-1 concentration since MCP-1 from samples and MCP-1-HRP conjugate compete for the anti-MCP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-1 from the sample, fewer sites are left to bind MCP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MCP-1 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 545465
• NCBI GeneID: 6347
• Molecular Weight: 11,025 Da
• NCBI Official Full Name: MCP-1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: CCL2
• NCBI Official Synonym Symbols: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• NCBI Protein Information: C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: Major capsid protein
• UniProt Gene Name: CCL2
• UniProt Synonym Gene Names: MCP1; SCYA2; MCAF; MCP-1
• UniProt Entry Name: CCL2_HUMAN

NCBI Description

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; protein kinase activity; receptor binding

Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; cellular homeostasis; negative regulation of neuron apoptosis; cell adhesion; neutrophil chemotaxis; organ regeneration; JAK-STAT cascade; positive regulation of tumor necrosis factor production; response to amino acid stimulus; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; unfolded protein response, activation of signaling protein activity; response to ethanol; cellular response to insulin stimulus; response to bacterium; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; response to wounding; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; leukocyte migration during inflammatory response; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To