Bovine Krueppel-like factor 9 (KLF9) ELISA Kit | KLF9 elisa kit

Bovine Krueppel-like factor 9 (KLF9) ELISA Kit

Cat. Num. AAA7242197

• Gene Names: KLF9; BTEB; BTEB1
• Reactivity: Bovine
• Synonyms: Krueppel-like factor 9 (KLF9); Bovine Krueppel-like factor 9 (KLF9) ELISA Kit; KLF9 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This KLF9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine KLF9. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLF9 elisa kit

Principle of the assay: KLF9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLF9 antibody and an KLF9-HRP conjugate. The assay sample and buffer are incubated together with KLF9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF9 concentration since KLF9 from samples and KLF9-HRP conjugate compete for the anti-KLF9 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF9 from the sample, fewer sites are left to bind KLF9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for KLF9 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4557375
• NCBI GeneID: 687
• NCBI Accession #: NP_001197.1
• NCBI GenBank Nucleotide #: NM_001206.2
• Molecular Weight: 27,235 Da
• NCBI Official Full Name: Krueppel-like factor 9
• NCBI Official Synonym Full Names: Kruppel-like factor 9
• NCBI Official Symbol: KLF9
• NCBI Official Synonym Symbols: BTEB; BTEB1
• NCBI Protein Information: Krueppel-like factor 9; BTE-binding protein 1; GC-box-binding protein 1; transcription factor BTEB1; basic transcription element binding protein 1; basic transcription element-binding protein 1
• UniProt Protein Name: Krueppel-like factor 9
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF9
• UniProt Synonym Gene Names: BTEB; BTEB1; BTE-binding protein 1
• UniProt Entry Name: KLF9_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

KLF9: Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 9q13

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; progesterone receptor signaling pathway; transcription, DNA-dependent; embryo implantation