Bovine Ficolin 3 (FCN3) ELISA Kit | FCN3 elisa kit

Bovine Ficolin 3 (FCN3) ELISA Kit

Cat. Num. AAA2800407

• Gene Names: FCN3; FCNH; HAKA1
• Reactivity: Bovine
• Synonyms: Ficolin 3 (FCN3); Bovine Ficolin 3 (FCN3) ELISA Kit; FCNH; HAKA1; MGC22543; H-ficolin; collagen/fibrinogen domain-containing lectin 3 p35; collagen/fibrinogen domain-containing protein 3; ficolin 3; FCN3 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine FCN3. No significant cross-reactivity or interference between Bovine FCN3 and analogues was observed.
• Sequence Length: 299

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for FCN3 elisa kit

This assay employs a two-site sandwich ELISA to quantitate FCN3 in samples. An antibody specific for FCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27754776
• NCBI GeneID: 8547
• NCBI Accession #: NP_003656.2
• NCBI GenBank Nucleotide #: NM_003665.3
• UniProt Accession #: O75636; Q6IBJ5; Q8WW86
• Molecular Weight: 31,678 Da
• NCBI Official Full Name: ficolin-3 isoform 1
• NCBI Official Synonym Full Names: ficolin 3
• NCBI Official Symbol: FCN3
• NCBI Official Synonym Symbols: FCNH; HAKA1
• NCBI Protein Information: ficolin-3
• UniProt Protein Name: Ficolin-3
• Protein Family: Ficolin
• UniProt Gene Name: FCN3
• UniProt Synonym Gene Names: FCNH; HAKA1

NCBI Description

Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.