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Typical Testing Data/Standard Curve (for reference only)

Mouse Ficolin 3 (FCN3) ELISA Kit | FCN3 elisa kit

Mouse Ficolin 3 (FCN3) ELISA Kit

Gene Names
FCN3; FCNH; HAKA1
Reactivity
Mouse
Synonyms
Ficolin 3 (FCN3); Mouse Ficolin 3 (FCN3) ELISA Kit; FCNH; HAKA1; Collagen/Fibrinogen Domain Containing; Hakata Antigen; Collagen/fibrinogen domain-containing lectin 3 p35; FCN3 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Ficolin 3 (FCN3).
No significant cross-reactivity or interference between Ficolin 3 (FCN3) and analogues was observed.
Sequence Length
299
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
< 6.9pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Ficolin 3 (FCN3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Ficolin 3 (FCN3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FCN3 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ficolin 3 (FCN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ficolin 3 (FCN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ficolin 3 (FCN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ficolin 3 (FCN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FCN3 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,678 Da
NCBI Official Full Name
ficolin-3 isoform 1
NCBI Official Synonym Full Names
ficolin 3
NCBI Official Symbol
FCN3
NCBI Official Synonym Symbols
FCNH; HAKA1
NCBI Protein Information
ficolin-3
UniProt Protein Name
Ficolin-3
Protein Family
UniProt Gene Name
FCN3
UniProt Synonym Gene Names
FCNH; HAKA1

NCBI Description

Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FCN3: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. Defects in FCN3 are the cause of ficolin 3 deficiency (FCN3D). FCN3D is a disorder characterized by immunodeficiency, recurrent infections, brain abscesses and recurrent warts on the fingers. Affected individuals have normal levels of lymphocytes, normal T-cell responses, and normal antibodies, but a selective deficient antibody response to pneumococcal polysaccharide vaccine. Belongs to the ficolin lectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: collagen; extracellular region

Molecular Function: antigen binding; carbohydrate binding; metal ion binding; protein binding; serine-type endopeptidase activity

Biological Process: complement activation; complement activation, lectin pathway; defense response to virus; negative regulation of virion penetration into host cell; proteolysis; recognition of apoptotic cell

Disease: Immunodeficiency Due To Ficolin 3 Deficiency

Research Articles on FCN3

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Product Notes

The Mouse FCN3 fcn3 (Catalog #AAA2097980) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2097980 ELISA Kit recognizes Mouse FCN3. It is sometimes possible for the material contained within the vial of "Ficolin 3 (FCN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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