Bovine Complement Component 4 (C4) ELISA Kit | C4 elisa kit

Bovine Complement Component 4 (C4) ELISA Kit

Cat. Num. AAA2608888

• Gene Names: C4BPA; PRP; C4BP
• Reactivity: Bovine
• Synonyms: Complement Component 4 (C4); Bovine Complement Component 4 (C4) ELISA Kit; C4 elisa kit

• Reactivity: Bovine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 597

• Samples: Bovine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ug/ml-1.56 ug/ml
• Sensitivity: Up to 0.5 ug/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine C4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 18490166
• NCBI GeneID: 722
• Molecular Weight: 67,033 Da
• NCBI Official Full Name: Complement component 4 binding protein, alpha
• NCBI Official Synonym Full Names: complement component 4 binding protein alpha
• NCBI Official Symbol: C4BPA
• NCBI Official Synonym Symbols: PRP; C4BP
• NCBI Protein Information: C4b-binding protein alpha chain
• UniProt Protein Name: C4b-binding protein alpha chain
• Protein Family: Complement C4
• UniProt Gene Name: C4BPA
• UniProt Synonym Gene Names: C4BP; C4bp; PRP
• UniProt Entry Name: C4BPA_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; plasma membrane

Molecular Function: protein binding

Biological Process: negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation