Typical Testing Data/Standard Curve (for reference only)
Bovine CD81 antigen (CD81) ELISA Kit | CD81 elisa kit
Bovine CD81 antigen (CD81) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CD81 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD81 antibody and an CD81-HRP conjugate. The assay sample and buffer are incubated together with CD81-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD81 concentration since CD81 from samples and CD81-HRP conjugate compete for the anti-CD81 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD81 from the sample, fewer sites are left to bind CD81-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD81 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
may play a role in glial scar formation in response to astrocyte injury; may be involved in regulation of cell interactions and cell growth [RGD, Feb 2006]
Uniprot Description
CD81: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the tetraspanin (TM4SF) family.
Protein type: Membrane protein, integral; Cell cycle regulation; Membrane protein, multi-pass
Cellular Component: focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; immunological synapse; vesicle
Molecular Function: protein binding
Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; protein localization; activation of MAPK activity; regulation of growth; positive regulation of cell proliferation; positive regulation of 1-phosphatidylinositol 4-kinase activity; positive regulation of B cell proliferation; response to wounding; phosphatidylinositol biosynthetic process; phosphatidylinositol metabolic process; regulation of cell proliferation
Research Articles on CD81
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Product Notes
The Bovine CD81 cd81 (Catalog #AAA7233742) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233742 ELISA Kit recognizes Bovine CD81. It is sometimes possible for the material contained within the vial of "CD81 antigen (CD81), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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