Mouse anti-Human MFAP4 Monoclonal Antibody | anti-MFAP4 antibody

Anti-MFAP4 Antibody (clone AT12D11) IHC-plus

Cat. Num. AAA249429

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A purified
• Synonyms: MFAP4; Monoclonal Antibody; Anti-MFAP4 Antibody (clone AT12D11) IHC-plus; Mouse Monoclonal [clone AT12D11] (IgG1; k) to Human MFAP4; Human MFAP4; anti-MFAP4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: AT12D11
• Specificity: Human MFAP4
• Purity/Purification: Protein A purified
• Form/Format: PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 255

• Applicable Applications for anti-MFAP4 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (10 ug/ml); WB (1:1000)
• Immunogen: MFAP4 antibody was raised against recombinant human MFAP4 (22-255aa) purified from E Coli.
• Immunogen Description: Recombinant human MFAP4 (22-255aa) purified from E Coli.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

Immunohistochemistry (IHC)

(Anti-MFAP4 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western Blot: The extract of lung (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MFAP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-MFAP4 antibody

MFAP4 Antibody Description: MFAP4 is a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 2506403
• NCBI GeneID: 4239
• UniProt Accession #: P55083; Q6P680; A8KAJ1; A8MVM2; B4E317
• NCBI Official Full Name: Microfibril-associated glycoprotein 4
• NCBI Official Synonym Full Names: microfibrillar associated protein 4
• NCBI Official Symbol: MFAP4
• NCBI Protein Information: microfibril-associated glycoprotein 4
• UniProt Protein Name: Microfibril-associated glycoprotein 4
• Protein Family: Microfibril-associated glycoprotein
• UniProt Gene Name: MFAP4
• UniProt Entry Name: MFAP4_HUMAN

NCBI Description

This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

MFAP4: Could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 is deleted in the Smith-Magenis syndrome (SMS).

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Calcium-binding

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: extracellular matrix; extracellular region; microfibril

Molecular Function: protein binding

Biological Process: cell adhesion; elastic fiber assembly; extracellular matrix organization and biogenesis; fibril organization and biogenesis; UV protection