Fibroblast Growth Factor-acidic Active Protein | baFGF active protein
Bovine Fibroblast Growth Factor-acidic
Gene Names
FGF1; ECGF; FGF-1
Purity
Greater than 90%.
Synonyms
Fibroblast Growth Factor-acidic; Bovine Fibroblast Growth Factor-acidic; aFGF Bovine; Fibroblast Growth Factor Acidic Bovine; HBGF-1; ECGF-beta; FIBP; FGFIBP; FIBP-1; ECGF; ECGFA; GLIO703; FGF1; FGF-a; baFGF active protein
Host
Bovine Brain
Purity/Purification
Greater than 90%.
Form/Format
Each 5 ug aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence Length
155
Solubility
It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.
Biological Activity
Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.
Preparation and Storage
Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18 degree C. Upon reconstitution aFGF should be stored at 4 degree C between 2-3 weeks and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Related Product Information for baFGF active protein
Description: Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al ). The FGF acidic is purified by proprietary chromatographic techniques.
Introduction: Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Introduction: Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Product Categories/Family for baFGF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,493 Da
NCBI Official Full Name
fibroblast growth factor 1
NCBI Official Synonym Full Names
fibroblast growth factor 1 (acidic)
NCBI Official Symbol
FGF1
NCBI Official Synonym Symbols
ECGF; FGF-1
NCBI Protein Information
fibroblast growth factor 1; EDGF II; HBGF-1; aFGF; acidic eye-derived growth factor II; acidic fibroblast growth factor; endothelial cell growth factor beta and alpha chains; endothelial growth factor; fibroblast growth factor, acidic; heparin-binding growth factor 1; prostatropin
UniProt Protein Name
Fibroblast growth factor 1
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
AFGF; FGFA; HBGF-1; FGF-1; EDGF II; aFGF; ECGF; HBGF-1
UniProt Entry Name
FGF1_BOVIN
Uniprot Description
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis ().
Research Articles on baFGF
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Product Notes
The baFGF fgf1 (Catalog #AAA143477) is an Active Protein produced from Bovine Brain and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor-acidic, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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