Typical Testing Data/Standard Curve (for reference only)
Mouse Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit | FGF1 elisa kit
Mouse Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit
Gene Names
FGF1; ECGF; EDGF; aFGF; FGF-1; HBGF-1; EDGF II
Reactivity
Mouse
Synonyms
Fibroblast Growth Factor 1; Acidic (FGF1); Mouse Fibroblast Growth Factor 1; Acidic (FGF1) ELISA Kit; FGF1 elisa kit
Reactivity
Mouse
Sequence Length
155
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
3ng/L - 900ng/L
Sensitivity
1.51ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FGF1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Fibroblast Growth Factor 1, Acidic (also known as FGF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse FGF1 antibody. FGF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FGF1 Antibody is added and binds to FGF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FGF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FGF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse FGF1 antibody. FGF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FGF1 Antibody is added and binds to FGF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FGF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FGF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,493 Da
NCBI Official Full Name
fibroblast growth factor 1
NCBI Official Synonym Full Names
fibroblast growth factor 1
NCBI Official Symbol
FGF1
NCBI Official Synonym Symbols
ECGF; EDGF; aFGF; FGF-1; HBGF-1; EDGF II
NCBI Protein Information
fibroblast growth factor 1
UniProt Protein Name
Fibroblast growth factor 1
Protein Family
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
AFGF; FGFA; HBGF-1; FGF-1; EDGF II; aFGF; ECGF; HBGF-1
Uniprot Description
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis ().
Research Articles on FGF1
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Product Notes
The Mouse FGF1 fgf1 (Catalog #AAA161312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161312 ELISA Kit recognizes Mouse FGF1. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 1, Acidic (FGF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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