Bone Morphogenetic Protein 7 Recombinant Protein | BMP7 recombinant protein

Recombinant Bone Morphogenetic Protein 7 (BMP7)

Cat. Num. AAA2029293

• Gene Names: BMP7; OP-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Bone Morphogenetic Protein 7; Recombinant Bone Morphogenetic Protein 7 (BMP7); BMP7 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 7.4 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- D FSLDNEVHSS FIHRRLRSQE RREMQREILS ILGLPHRPRP HLQGKHNSAP MFMLDLYNAM AVEEGGGPGG QGFSYPYKAV FSTQGPPLAS LQDSHFLTDA DMVMSFVNLV EHDKEFFHPR YHHREFRFDL SKIPEGEAVT AAEFRIYKDY IRERFDNETF RISVYQVLQE HLGRESDLFL LDSRTLWASE EGWLVFDITA TSNHWVVNPR HNLGLQLSVE TLDGQSINPK LAGLIGRHGP QNKQPFMVAF FKATEVHFRS IRSTGSKQRS QNRSKTPKNQ EALRMANVAE NSSSDQRQAC KKHELYVSFR DLGWQDWIIA PEGYAAYYCE GECAFPLNSY MNATNHAIVQ TLVHFINPET VPKPCCAPTQ LNAISVLYFDDSSNVILKKY RNMVVRACGC H
• Sequence Length: 431

• Applicable Applications for BMP7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 49.7kDa
• Accurate Molecular Mass (KD): 44kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala25~Lys96
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4502427
• NCBI GeneID: 655
• NCBI Accession #: NP_001710.1
• NCBI GenBank Nucleotide #: NM_001719.2
• Molecular Weight: 49,313 Da
• NCBI Official Full Name: bone morphogenetic protein 7
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP7
• UniProt Synonym Gene Names: OP1; BMP-7; OP-1
• UniProt Entry Name: BMP7_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: axon guidance; response to peptide hormone stimulus; negative regulation of MAP kinase activity; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; BMP signaling pathway; regulation of apoptosis; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; mesoderm formation; branching morphogenesis of a tube; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; epithelial to mesenchymal transition; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; neurite morphogenesis; growth