Bone Morphogenetic Protein 4 (BMP4) Recombinant Protein | BMP4 recombinant protein

Recombinant Bone Morphogenetic Protein 4 (BMP4)

Cat. Num. AAA2009351

• Gene Names: Bmp4; BOMPR4A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Bone Morphogenetic Protein 4 (BMP4); Recombinant Bone Morphogenetic Protein 4 (BMP4); BMP4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- PET GKKKVAEIQG HAGGRRSGQS HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEEQS QGTGLEYPER PASRANTVRS FHHEEHLENI PGTSESSAFR FFFNLSSIPE NEVISSAELR LFREQVDQGP DWEQGFHRIN IYE
• Sequence Length: 408

• Applicable Applications for BMP4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Pro28~Glu183 (Accession # Q06826) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 543902
• NCBI GeneID: 25296
• UniProt Accession #: Q06826
• Molecular Weight: 19.5kDa
• NCBI Official Full Name: Bone morphogenetic protein 4
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: BOMPR4A
• NCBI Protein Information: bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_RAT

NCBI Description

plays a role in induction of cell proliferation [RGD, Feb 2006]

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: heparin binding; protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity

Biological Process: negative regulation of MAP kinase activity; activation of MAPKK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; embryonic skeletal development; negative regulation of chondrocyte differentiation; mesodermal cell differentiation; telencephalon regionalization; germ cell development; regulation of protein import into nucleus; BMP signaling pathway; anatomical structure formation; mesonephros development; kidney development; endochondral ossification; regulation of odontogenesis of dentine-containing teeth; embryonic limb morphogenesis; positive regulation of cardiac muscle fiber development; negative regulation of immature T cell proliferation in the thymus; tongue morphogenesis; cell fate commitment; camera-type eye development; regulation of smooth muscle cell proliferation; neuron fate commitment; response to testosterone stimulus; camera-type eye morphogenesis; regulation of gene expression; retina development in camera-type eye; response to mechanical stimulus; negative regulation of mitosis; positive regulation of epidermal cell differentiation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of apoptosis; tissue development; wound healing; positive regulation of protein binding; cloacal septation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; negative regulation of cell proliferation; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; forebrain development; positive regulation of cell proliferation; angiogenesis; embryonic morphogenesis; positive regulation of BMP signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; embryonic skeletal morphogenesis; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; cell proliferation; blood vessel endothelial cell proliferation during sprouting angiogenesis; telencephalon development; ureteric bud branching; regulation of cell fate commitment; brain development; positive regulation of neuron differentiation; anterior/posterior axis specification; lung development; renal system process; macrophage differentiation; heart development; response to glucocorticoid stimulus; lymphoid progenitor cell differentiation; response to organic cyclic substance; post-embryonic development; positive regulation of endothelial cell differentiation; positive chemotaxis; induction of an organ; erythrocyte differentiation; chondrocyte differentiation; mesoderm development; specification of organ position; monocyte differentiation; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; mesoderm formation; branching morphogenesis of a tube; negative regulation of phosphorylation; positive regulation of endothelial cell proliferation; hemopoietic progenitor cell differentiation; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; metanephros development; alveolus development; positive regulation of epithelial cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; embryonic hindlimb morphogenesis; response to estradiol stimulus; negative regulation of cell cycle; odontogenesis; smooth muscle development; vasculature development; ovarian follicle development; regulation of cell differentiation; regulation of smooth muscle cell differentiation; skeletal development; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; blood vessel development; smoothened signaling pathway; response to retinoic acid; negative regulation of oligodendrocyte differentiation; eye development; pituitary gland development; neural tube closure; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; mesodermal cell fate determination; growth