Bone Morphogenetic protein-2 Recombinant Protein | BMP-2 recombinant protein

Human Bone Morphogenetic protein-2 Recombinant

Cat. Num. AAA553031

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Bone Morphogenetic protein-2; Human Bone Morphogenetic protein-2 Recombinant; bmp2; bone morphogenetic protein; bmp-2; BMP-2 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Human Bone Morphogenetic Protein-2 was lyophilized from a 0.2 um filtered solution in 20mM AcOH pH 6.5.
• Sequence: MVAGTRCLLA LLLPQVLLGG AAGLVPELGR RKFAAASSGR PSSQPSDEVL SEFELRLLSM FGLKQRPTPS RDAVVPPYML DLYRRHSGQP GSPAPDHRLE RAASRANTVR SFHHEESLEE LPETSGKTTR RFFFNLSSIP TEEFITSAEL QVFREQMQDA LGNNSSFHHR INIYEIIKPA TANSKFPVTR LLDTRLVNQN ASRWESFDVT PAVMRWTAQG HANHGFVVEV AHLEEKQGVS KRHVRISRSL HQDEHSWSQI RPLLVTFGHD GKGHPLHKRE KRQAKHKQRK RLKSSCKRHP LYVDFSDVGW NDWIVAPPGY HAFYCHGECP FPLADHLNST NHAIVQTLVN SVNSKIPKAC CVPTELSAIS MLYLDENEKV VLKNYQDMVV EGCGCR
• Sequence Length: 396

• Domain: Transforming growh factor beta
• Host Note: Optimized DNA sequence encoding Human Bone Morphogenetic Protein-2 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Bone Morphogenetic Protein-2 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.; ; Recombinant BMP-2 is a disulfide-linked homodimeric protein consisting of two 114 amino acid residue subunits. BMP2 migrates as an approximately 25 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for BMP-2 recombinant protein

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP-2 wild type binds to its cellular receptors via two distinct binding epitopes. The large epitope 1 is responsible for the high- affinity binding to the BMPR-IA receptor, the smaller epitope 2 provides the low-affinity binding to the receptor BMPR-II.

Product Categories/Family for BMP-2 recombinant protein

Cytokines

References

• recombinant human bmp-2 enhances local bone formation in the lumbar spine of osteoporotic sheep .J Bone Joint Surg Br Proceedings, Sep 2009; 91-B: 436.
• remodeling of chromatin structure within the promoter is important for bmp-2-induced fgfr3 expression .Nucleic Acids Res., Jul 2009; 37: 3897 - 3911.
• bmp-2 is a cost-effective therapy in grade iii open tibia fractures – a health-economic assessment of the use bmp-2 in open tibia fractures for european health care systems .J Bone Joint Surg Br Proceedings, Mar 2009; 91-B: 155.
• activation of amp kinase and inhibition of rho kinase induce the mineralization of osteoblastic mc3t3-e1 cells through endothelial nos and bmp-2 expression .Am J Physiol Endocrinol Metab, Jan 2009; 296: E139 - E146.
• irbesartan attenuates ang ii-induced bmp-2 expression in human umbilical vein endothelial cells .Vascular Medicine, Aug 2008; 13: 239 - 245.