Bone Morphogenetic Protein 2 (BMP2) Recombinant Protein | BMP2 recombinant protein

Recombinant Bone Morphogenetic Protein 2 (BMP2)

Cat. Num. AAA2012162

• Gene Names: Bmp2; Bmp2a; AI467020
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Bone Morphogenetic Protein 2 (BMP2); Recombinant Bone Morphogenetic Protein 2 (BMP2); BMP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGS-QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
• Sequence Length: 394

• Applicable Applications for BMP2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln281~Arg394 (Accession # P21274) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 71896669
• NCBI GeneID: 12156
• NCBI Accession #: NP_031579.2
• NCBI GenBank Nucleotide #: NM_007553.3
• UniProt Accession #: P21274; Q497W8
• Molecular Weight: 14.1kDa
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: Bmp2
• NCBI Official Synonym Symbols: Bmp2a; AI467020
• NCBI Protein Information: bone morphogenetic protein 2; bone morphogenetic protein 2A
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp2
• UniProt Synonym Gene Names: Bmp-2; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_MOUSE

NCBI Description

This gene encodes a member of a family of secreted factors that participate in developmental signal transduction to stimulate SMAD transcriptional activators. The encoded protein is able to induce cartilage and bone formation. The propeptide is further cleaved into an active chain. [provided by RefSeq, Mar 2013]

Uniprot Description

BMP2: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular space; protein complex; cell surface; cytoplasm; extracellular region

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; phosphatase activator activity; cytokine activity; SMAD binding; transforming growth factor beta receptor binding; receptor binding; retinol dehydrogenase activity

Biological Process: activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; heart development; multicellular organismal development; adrenocorticotropin hormone secreting cell differentiation; telencephalon regionalization; protein amino acid phosphorylation; cardiac muscle cell differentiation; regulation of apoptosis; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; chondrocyte differentiation; inner ear development; regulation of odontogenesis of dentine-containing teeth; positive regulation of astrocyte differentiation; pericardium development; positive regulation of neurogenesis; cell fate commitment; organ morphogenesis; mesenchymal cell differentiation; regulation of MAPKKK cascade; positive regulation of osteoblast proliferation; positive regulation of fat cell differentiation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; negative regulation of calcium-independent cell-cell adhesion; positive regulation of odontogenesis; proteoglycan metabolic process; embryonic heart tube anterior/posterior pattern formation; negative regulation of insulin-like growth factor receptor signaling pathway; thyroid stimulating hormone secreting cell differentiation; negative regulation of transcription from RNA polymerase II promoter; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; cardiac muscle morphogensis; negative regulation of aldosterone biosynthetic process; cell differentiation; cardiac cell differentiation; positive regulation of Wnt receptor signaling pathway; Notch signaling pathway; ossification; protein destabilization; in utero embryonic development; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; telencephalon development; embryonic development; ureteric bud branching; cartilage development; response to hypoxia; epithelial to mesenchymal transition; positive regulation of protein amino acid phosphorylation; positive regulation of neuron differentiation; positive regulation of cell migration; growth