Mouse bone morphogenetic protein 2 ELISA Kit | BMP2 elisa kit

Mouse Bone morphogenetic protein 2, BMP-2 ELISA Kit

Cat. Num. AAA701468

• Gene Names: Bmp2; Bmp2a; AI467020
• Reactivity: Mouse
• Synonyms: bone morphogenetic protein 2; Mouse Bone morphogenetic protein 2; BMP-2 ELISA Kit; BMP2A; ; BMP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse BMP2. No significant cross-reactivity or interference between Mouse BMP2 and analogues was observed.
• Sequence Length: 394

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 1.56 pg/ml-100 pg/ml
• Sensitivity: 0.39 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Chondrogenesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP2 elisa kit

Introduction: Bone morphogenetic protein 2 or BMP-2 belongs to the TGF-beta superfamily of proteins. Like other bone morphogenetic proteins, BMP-2 plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. Members of the BMP family are potentially useful as therapeutics in areas such as spinal fusion. BMP-2 and BMP-7 have been shown in clinical studies to be beneficial in the treatment of a variety of bone-related conditions including delayed union and non-union.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BMP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BMP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BMP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71896669
• NCBI GeneID: 12156
• NCBI Accession #: NP_031579.2
• NCBI GenBank Nucleotide #: NM_007553.3
• UniProt Accession #: P21274; Q497W8
• Molecular Weight: 44,525 Da
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: Bmp2
• NCBI Official Synonym Symbols: Bmp2a; AI467020
• NCBI Protein Information: bone morphogenetic protein 2; bone morphogenetic protein 2A
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp2
• UniProt Synonym Gene Names: Bmp-2; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_MOUSE

NCBI Description

This gene encodes a member of a family of secreted factors that participate in developmental signal transduction to stimulate SMAD transcriptional activators. The encoded protein is able to induce cartilage and bone formation. The propeptide is further cleaved into an active chain. [provided by RefSeq, Mar 2013]

Uniprot Description

Function: Induces cartilage and bone formation.