Biliverdin Reductase A Recombinant Protein | BLVRA recombinant protein

Recombinant Biliverdin Reductase A (BLVRA)

Cat. Num. AAA2031537

• Gene Names: BLVRA; BVR; BLVR; BVRA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Biliverdin Reductase A; Recombinant Biliverdin Reductase A (BLVRA); BLVRA recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MNAEPERKFG VVVVGVGRAG SVRMRDLRNP HPSSAFLNLI GFVSRRELGSIDGVQQISLE DALSSQEVEV AYICSESSSH EDYIRQFLNA GKHVLVEYPMTLSLAAAQEL WELAEQKGKV LHEEH
• Sequence Length: 296

• Applicable Applications for BLVRA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 17.6kDa
• Accurate Molecular Mass (KD): 18kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~His125
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 359465572
• NCBI GeneID: 644
• NCBI Accession #: NP_001240752.1
• NCBI GenBank Nucleotide #: NM_001253823.1
• Molecular Weight: 33,428 Da
• NCBI Official Full Name: biliverdin reductase A
• NCBI Official Synonym Full Names: biliverdin reductase A
• NCBI Official Symbol: BLVRA
• NCBI Official Synonym Symbols: BVR; BLVR; BVRA
• NCBI Protein Information: biliverdin reductase A
• UniProt Protein Name: Biliverdin reductase A
• Protein Family: Biliverdin reductase
• UniProt Gene Name: BLVRA
• UniProt Synonym Gene Names: BLVR; BVR; BVR A
• UniProt Entry Name: BIEA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the biliverdin reductase family, members of which catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. Mutations in this gene are associated with hyperbiliverdinemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

BVR: Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Monomer. Liver. Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.3.1.24; Oxidoreductase; Kinase, protein; ATYPICAL group; BLVRA family

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cytosol

Molecular Function: zinc ion binding; biliverdin reductase activity

Biological Process: heme catabolic process; porphyrin metabolic process

Disease: Hyperbiliverdinemia