USP14 blocking peptide

USP14 Blocking Peptide (C-term)

• Gene Names: USP14; TGT
• Synonyms: USP14; USP14 Blocking Peptide (C-term); Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; TGT; USP14 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 370-383 of HUMAN USP14
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 494

• Cellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for USP14 blocking peptide

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).

NCBI and Uniprot Product Information

• NCBI GI #: 4827050
• NCBI GeneID: 9097
• NCBI Accession #: NP_005142.1
• NCBI GenBank Nucleotide #: NM_005151.3
• UniProt Accession #: P54578; Q53XY5; B7Z4N8; J3QRZ5
• Molecular Weight: 54,770 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 14 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 14
• NCBI Official Symbol: USP14
• NCBI Official Synonym Symbols: TGT
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 14
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 14
• UniProt Gene Name: USP14
• UniProt Synonym Gene Names: TGT
• UniProt Entry Name: UBP14_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: cell surface; cytoplasm; cytoplasmic membrane-bound vesicle

Molecular Function: cysteine-type endopeptidase activity; endopeptidase inhibitor activity; protein binding; tRNA guanylyltransferase activity; ubiquitin-specific protease activity

Biological Process: protein deubiquitination; regulation of chemotaxis