Western Blot (WB)
(Western blotAll lanes: Ubiquitin carboxyl-terminal hydrolase 14 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateLane 3:NIH3T3 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 57,53,55 kDaObserved band size: 56 kDa)
Rabbit anti-Human Ubiquitin carboxyl-terminal hydrolase 14 Polyclonal Antibody | anti-USP14 antibody
Rabbit anti-human Ubiquitin carboxyl-terminal hydrolase 14 polyclonal Antibody
Western Blot (WB)
(Western blotAll lanes: Ubiquitin carboxyl-terminal hydrolase 14 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateLane 3:NIH3T3 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 57,53,55 kDaObserved band size: 56 kDa)
Western Blot (WB)
(Western blotAll lanes: Ubiquitin carboxyl-terminal hydrolase 14 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateLane 3:NIH3T3 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 57,53,55 kDaObserved band size: 56 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7001109 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7001109 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7001109 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7001109 at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells cells using MBS7001109 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells cells using MBS7001109 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
Protein type: Ubiquitin conjugating system; EC 3.4.19.12; Protease; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 18p11.32
Cellular Component: cell surface; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; proteasome complex; synapse
Molecular Function: cysteine-type endopeptidase activity; endopeptidase inhibitor activity; protein binding; tRNA guanylyltransferase activity; ubiquitin-specific protease activity
Biological Process: protein deubiquitination; regulation of chemotaxis; synaptic transmission; ubiquitin-dependent protein catabolic process
Research Articles on USP14
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Product Notes
The USP14 usp14 (Catalog #AAA7001109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Ubiquitin carboxyl-terminal hydrolase 14 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin carboxyl-terminal hydrolase 14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the USP14 usp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin carboxyl-terminal hydrolase 14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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