USO1 blocking peptide

USO1 Antibody (C-term) Blocking peptide

• Gene Names: USO1; TAP; VDP; P115
• Synonyms: USO1; USO1 Antibody (C-term) Blocking peptide; General vesicular transport factor p115; Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein; VDP; USO1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of USO1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 962

• Cellular Location: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for USO1 blocking peptide

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 143743108
• NCBI GeneID: 8615
• UniProt Accession #: O60763; Q6PK63; Q86TB8; Q8N592; B2RAQ0
• Molecular Weight: 109,195 Da
• NCBI Official Full Name: General vesicular transport factor p115
• NCBI Official Synonym Full Names: USO1 vesicle transport factor
• NCBI Official Symbol: USO1
• NCBI Official Synonym Symbols: TAP; VDP; P115
• NCBI Protein Information: general vesicular transport factor p115
• UniProt Protein Name: General vesicular transport factor p115
• Protein Family: General vesicular transport factor
• UniProt Gene Name: USO1
• UniProt Synonym Gene Names: VDP; TAP
• UniProt Entry Name: USO1_HUMAN

NCBI Description

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: cytosol; endoplasmic reticulum; Golgi apparatus; Golgi membrane; Golgi stack; membrane; nucleolus; transport vesicle

Molecular Function: protein binding; protein transporter activity

Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; intracellular protein transport; transcytosis