URAT1 blocking peptide

URAT1 Blocking Peptide

Cat. Num. AAA9621045

• Gene Names: SLC22A12; RST; OAT4L; URAT1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: URAT1; URAT1 Blocking Peptide; Organic anion transporter 4-like protein; Renal-specific transporter; RST; S22AC_HUMAN; Slc22a12; Solute carrier family 22 member 12; Urate anion exchanger 1; URAT1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for URAT1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for URAT1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 444299636
• NCBI GeneID: 116085
• NCBI Accession #: NP_001263255.1
• NCBI GenBank Nucleotide #: NM_001276326.1
• UniProt Accession #: Q96S37; Q19PF7; Q19PF8; Q19PF9; Q19PG0; Q6UXW3; Q96DT2; B7WPG1; G3XAN7
• Molecular Weight: 56,094 Da
• NCBI Official Full Name: solute carrier family 22 member 12 isoform c
• NCBI Official Synonym Full Names: solute carrier family 22 member 12
• NCBI Official Symbol: SLC22A12
• NCBI Official Synonym Symbols: RST; OAT4L; URAT1
• NCBI Protein Information: solute carrier family 22 member 12
• UniProt Protein Name: Solute carrier family 22 member 12
• UniProt Gene Name: SLC22A12
• UniProt Synonym Gene Names: OATL4; URAT1; RST
• UniProt Entry Name: S22AC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the organic anion transporter (OAT) family, and it acts as a urate transporter to regulate urate levels in blood. This protein is an integral membrane protein primarily found in epithelial cells of the proximal tubule of the kidney. An elevated level of serum urate, hyperuricemia, is associated with increased incidences of gout, and mutations in this gene cause renal hypouricemia type 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

SLC22A12: Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions. Defects in SLC22A12 are the cause of hypouricemia renal type 1 (RHUC1). A disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion. Patients often appear asymptomatic, but may be subject to exercise-induced acute renal failure, chronic renal dysfunction and nephrolithiasis. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: urate transmembrane transporter activity; PDZ domain binding

Biological Process: response to drug; urate transport; urate metabolic process; cellular homeostasis; organic acid transport; transmembrane transport

Disease: Hypouricemia, Renal, 1