Trim24 blocking peptide

Trim24 Blocking Peptide (C-term)

• Gene Names: Trim24; TIF1; Tif1a; AI447469; TIF1alpha; TIF1-alpha; A130082H20Rik; D430004I05Rik
• Synonyms: Trim24; Trim24 Blocking Peptide (C-term); Transcription intermediary factor 1-alpha; TIF1-alpha; 632-; E3 ubiquitin-protein ligase Trim24; Tripartite motif-containing protein 24; Tif1; Tif1a; Trim24 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 789-823 of HUMAN Trim24
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1051

• Cellular Location: Nucleus. Cytoplasm. Note: Detected in the cytoplasm of the zygote. Translocates into the pronucleus at the time of genome activation. Colocalizes with sites of active transcription
• Tissue Location: Detected in embryonic and adult liver. Detected in zygote and throughout embryogenesis (at protein level). Detected in all adult tissues, with the highest expression level in testis.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Trim24 blocking peptide

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac) (By similarity). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis via its effects on p53/TP53 levels. Up-regulates ligand- dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, such as RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Required for normal transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 6175058
• NCBI GeneID: 21848
• UniProt Accession #: Q64127; Q64126
• Molecular Weight: 112,844 Da
• NCBI Official Full Name: Transcription intermediary factor 1-alpha
• NCBI Official Synonym Full Names: tripartite motif-containing 24
• NCBI Official Symbol: Trim24
• NCBI Official Synonym Symbols: TIF1; Tif1a; AI447469; TIF1alpha; TIF1-alpha; A130082H20Rik; D430004I05Rik
• NCBI Protein Information: transcription intermediary factor 1-alpha
• UniProt Protein Name: Transcription intermediary factor 1-alpha
• Protein Family: Transcription intermediary factor
• UniProt Gene Name: Trim24
• UniProt Synonym Gene Names: Tif1; Tif1a; TIF1-alpha
• UniProt Entry Name: TIF1A_MOUSE

NCBI Description

The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

TRIM24: an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55. Belongs to a family of nuclear proteins that includes TIF1b and TIF1g. TIF1 proteins are characterized by an N-terminal region that contains a RING finger-B boxes-coiled coil (RBCC) motif, a poorly conserved central region, a C-terminal region that contains a PHD finger, and a bromodomain. Interacts with the heterochromatin-associated proteins HP1a, MOD1 (HP1b), and MOD2 (HP1g) and with the transcriptional repression domain KRAB, which is present in many Kruppel-type (C2H2) zinc finger proteins. May act as a repressor through the formation of transcriptionally inactive heterochromatin. Two splice variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, atypical; EC 6.3.2.-; Tumor suppressor; Ubiquitin conjugating system; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family

Cellular Component: cytoplasm; nucleolus; nucleus; perichromatin fibrils

Molecular Function: chromatin binding; estrogen response element binding; ligand-dependent nuclear receptor binding; p53 binding; protein binding; protein kinase activity; sequence-specific DNA binding; transcription coactivator activity; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: calcium ion homeostasis; negative regulation of cell proliferation; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein catabolic process; protein ubiquitination; regulation of apoptosis; regulation of protein stability