TRAF3 blocking peptide

TRAF3 Antibody (C-term) Blocking peptide

• Gene Names: TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• Synonyms: TRAF3; TRAF3 Antibody (C-term) Blocking peptide; TNF receptor-associated factor 3; 632-; CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1; CAP1; TRAF3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of TRAF3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 568

• Cellular Location: Cytoplasm. Endosome. Mitochondrion. Note: Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TRAF3 blocking peptide

Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.

NCBI and Uniprot Product Information

• NCBI GI #: 116242824
• NCBI GeneID: 7187
• UniProt Accession #: Q13114; Q12990; Q13076; Q13947; Q6AZX1; Q9UNL1; B7Z8C4
• Molecular Weight: 55,306 Da
• NCBI Official Full Name: TNF receptor-associated factor 3
• NCBI Official Synonym Full Names: TNF receptor associated factor 3
• NCBI Official Symbol: TRAF3
• NCBI Official Synonym Symbols: CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• NCBI Protein Information: TNF receptor-associated factor 3
• UniProt Protein Name: TNF receptor-associated factor 3
• Protein Family: TRAF3-interacting protein
• UniProt Gene Name: TRAF3
• UniProt Synonym Gene Names: CAP1; CRAF1; CRAF1; CD40BP; LAP1
• UniProt Entry Name: TRAF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq, Dec 2010]

Uniprot Description

TRAF3: Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. Homotrimer. Heterotrimer with TRAF2 and TRAF5. Interacts with LTBR/TNFRSF3, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF13C TNFRSF17/BCMA, TLR4 and EDAR. Interacts with MAP3K5, MAP3K14, TRAIP/TRIP, TDP2/TTRAP, TANK/ITRAF and TRAF3IP1. Interaction with TNFRSF5/CD40 is modulated by TANK/ITRAF, which competes for the same binding site. Interacts with TICAM1. Interacts with TRAFD1. Interacts with OTUB1, OTUB2 and OTUD5. Interacts with RNF216, MAVS, OPTN and TBK1. Identified in a complex with TRAF2, MAP3K14 and BIRC3. Interacts with BIRC2 and BIRC3. Upon exposure to bacterial lipopolysaccharide (LPS), recruited to a transient complex containing TLR4, TRAF3, TRAF6, IKBKG, MAP3K7, MYD88, TICAM1, BIRC2, BIRC3 and UBE2N. Interacts with Epstein-Barr virus protein LMP1. Interacts (via RING-type zinc finger domain) with SRC. Belongs to the TNF receptor-associated factor family. A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: cytosol; internal side of plasma membrane

Molecular Function: protein binding; protein phosphatase binding; signal transducer activity; thioesterase binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of interferon type I production; regulation of cytokine production; regulation of defense response to virus; regulation of interferon-beta production; regulation of proteolysis; signal transduction; toll-like receptor signaling pathway; tumor necrosis factor-mediated signaling pathway

Disease: Herpes Simplex Encephalitis, Susceptibility To, 3