Serpin E1 Active Protein | SERPINE1 active protein

Recombinant Human Serpin E1 Protein

Cat. Num. AAA9139964

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Purity: >95% by SDS-PAGE.
• Synonyms: Serpin E1; Recombinant Human Serpin E1 Protein; Plasminogen Activator Inhibitor 1; PAI; PAI-1; Endothelial Plasminogen Activator Inhibitor; SerpinE1; SERPINE1; PAI1; PLANH1; SERPINE1 active protein

• Host: Human Cells
• Purity/Purification: >95% by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0.
• Sequence: VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILS
• Sequence Length: 402

• Species: Human
• Endotoxin: < 1 EU/ug of the protein by LAL method.
• Biological Activity: Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin. The IC50 value is < 60 nM.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
• Tag: 6xHis tag at the C-terminus
• Preparation and Storage: Store the lyophilized protein at -20 degree C to -80 degree C for long term.; After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.

SDS-Page

(Recombinant Human Serpin E1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.)

Related Product Information for SERPINE1 active protein

Description: Recombinant Human Serpin E1 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Val24-Pro402) of human Serpin E1 (Accession #P05121) fused with a 6xHis tag at the C-terminus.

Background: Serpins are a group of proteins with similar structures that were first identified as a set of proteins able toinhibit proteases. They are the largest and most diverse family of serine protease inhibitors which are involvedin a number of fundamental biological processes such as blood coagulation, complement activation,fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.Serpin E1 is a secreted protein which belongs to the Serpin family. Serpin E1 acts as 'bait' for tissueplasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major controlpoint in the regulation of fibrinolysis. Defects in SERPINE1 are characterized by abnormal bleeding due toSerpin E1 defect in the plasma. High concentrations of Serpin E1 have been associated with thrombophiliawhich is an autosomal dominant disorder in which affected individuals are prone to develop seriousspontaneous thrombosis.

Product Categories/Family for SERPINE1 active protein

Other Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NP_000593.1
• UniProt Accession #: P05121
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin family E member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• Protein Family: Plasminogen activator inhibitor
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; platelet degranulation; negative regulation of fibrinolysis; transforming growth factor beta receptor signaling pathway; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; platelet activation; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; positive regulation of angiogenesis; fibrinolysis; positive regulation of transcription from RNA polymerase II promoter; gene expression; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency