TPR blocking peptide

TPR Blocking Peptide (C-term)

• Synonyms: TPR; TPR Blocking Peptide (C-term); Nucleoprotein TPR; Megator; NPC-associated intranuclear protein; Translocated promoter region protein; TPR blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 2026-2040 of HUMAN TPR
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 2363

• Cellular Location: Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Nucleus envelope. Nucleus, nuclear pore complex. Cytoplasm. Cytoplasm, cytoskeleton, spindle Chromosome, centromere, kinetochore. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Detected as discrete intranuclear foci with IFI204 (By similarity). In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Detected exclusively to the cytoplasmic margin of NPC. Docking to the inner nucleoplasmic side of the NPC is mediated through binding to nucleoporins. Anchored by NUP153 to the NPC. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected as filaments that emanate from the nuclear basket of the NPC and extend to the nucleolus to delineate a chromatin-free network extending from the nuclear envelope to the perinucleolar region. Detected in diffuse and discrete spheroidal intranuclear foci. Nucleocytoplasmic shuttling protein imported into the nucleus in a XPO1/CRM1- and Importin alpha/Importin beta receptor-dependent manner. Remains localized to the nuclear membrane after poliovirus (PV) infection. During mitosis, remains associated with the nuclear envelope until prometaphase. Associated with the mitotic spindle from late prometaphase until anaphase. Reorganized during mitosis in a viscous and dynamic nuclear-derived spindle matrix that embeds the microtubule spindle apparatus from pole to pole in a microtubule- independent manner. Recruited to the reforming nuclear envelope during telophase and cytokinesis. Detected at kinetochores during prometaphase. Colocalizes with MAD2L1 in the spindle matrix but not at kinetochore. Colocalizes with dynein, dynactin, tubulin at kinetochore during the metaphase-anaphase transition. Colocalizes with DYNLL1 at the mitotic spindle.
• Tissue Location: Expressed in esophagus, ovary, liver, skin, smooth muscles, cerebrum and fetal cerebellum (at protein level) Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TPR blocking peptide

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. According to some authors, plays a limited role in the regulation of nuclear protein export (PubMed:22253824 and PubMed:11952838). Plays also a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases.

NCBI and Uniprot Product Information

• NCBI GI #: 215274208
• NCBI GeneID: 7175
• UniProt Accession #: P12270; Q15624; Q15655; Q5SWY0; Q99968
• Molecular Weight: 83,979 Da
• NCBI Official Full Name: Nucleoprotein TPR
• NCBI Official Synonym Full Names: translocated promoter region, nuclear basket protein
• NCBI Official Symbol: TPR
• NCBI Protein Information: nucleoprotein TPR
• UniProt Protein Name: Nucleoprotein TPR
• Protein Family: Thiol protease
• UniProt Gene Name: TPR
• UniProt Entry Name: TPR_HUMAN

NCBI Description

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008]

Uniprot Description

TPR: a large coiled-coil nuclear pore protein that forms filamentous structures extending from nuclear pore complexes (NPCs) into the perinucleolar region. Together with Lamina-associated polypeptide 2 (LAP2) family members and Nup153, forms structural links between the nuclear lamina and the internal nuclear matrix. In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus. Involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Translocations between human TPR and TrkA, MET, and RAF genes are oncogenic. Its N-terminus is involved in activation of oncogenic kinases.

Protein type: Nucleoporin; Oncoprotein; Membrane protein, peripheral; Nuclear envelope

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytoplasm; cytoplasmic dynein complex; extrinsic to membrane; kinetochore; nuclear envelope; nuclear inclusion body; nuclear membrane; nuclear pore; nucleoplasm; nucleus

Molecular Function: chromatin binding; heat shock protein binding; mitogen-activated protein kinase binding; mRNA binding; nucleocytoplasmic transporter activity; protein anchor; protein binding; protein homodimerization activity; transporter activity; tubulin binding

Biological Process: mitotic cell cycle spindle assembly checkpoint; mitotic nuclear envelope disassembly; mRNA export from nucleus; mRNA export from nucleus during heat stress; negative regulation of RNA export from nucleus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of translational initiation; nuclear matrix organization and biogenesis; nuclear pore complex assembly; nuclear pore organization and biogenesis; nuclear translocation of MAPK; positive regulation of heterochromatin formation; positive regulation of protein export from nucleus; positive regulation of protein import into nucleus; protein export from nucleus; protein import into nucleus; protein sumoylation; regulation of protein export from nucleus; regulation of protein import into nucleus; regulation of protein stability; RNA export from nucleus; RNA import into nucleus; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription