Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein PML (PML) Standard Curve.)
Mouse Protein PML (PML) ELISA Kit | PML elisa kit
Mouse Protein PML (PML) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein PML (PML) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein PML (PML) Standard Curve.)
Background: Probable transcription factor PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies (Nuclear dots) where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
NCBI and Uniprot Product Information
Uniprot Description
PML: a zinc-finger protein that can regulate transcription and can localize to nuclear bodies. Cytoplasmic forms regulate glycolysis by inhibiting the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Sumoylated forms interact with SATB1 and localize to the PML nuclear bodies. Sumoylation on three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The B1 box and the RING finger are also required for localization to nuclear bodies. May play an important role in recruitment of ELF4 into PML nuclear bodies. A chromosomal aberration involving PML can cause acute promyelocytic leukemia (APL). Seven alternatively-spliced human isoforms have been reported.
Protein type: Nucleolus; Oncoprotein; Transcription factor; Tumor suppressor; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 9 B|9 31.63 cM
Cellular Component: cytoplasm; cytosol; nuclear chromosome, telomeric region; nuclear matrix; nuclear membrane; nucleolus; nucleoplasm; nucleus; PML body
Molecular Function: protein binding; protein homodimerization activity; SMAD binding; SUMO binding; transcription coactivator activity; ubiquitin protein ligase binding
Biological Process: caspase activation; cell aging; cell cycle arrest; cell fate commitment; circadian regulation of gene expression; common-partner SMAD protein phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; endoplasmic reticulum calcium ion homeostasis; entrainment of circadian clock by photoperiod; induction of apoptosis by oxidative stress; innate immune response; maintenance of protein localization in nucleus; myeloid cell differentiation; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of interleukin-1 beta secretion; negative regulation of interleukin-1 secretion; negative regulation of transcription, DNA-dependent; PML body organization and biogenesis; positive regulation of fibroblast proliferation; positive regulation of MHC class I biosynthetic process; positive regulation of telomere maintenance; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; regulation of cell adhesion; regulation of circadian rhythm; regulation of MHC class I biosynthetic process; regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to gamma radiation; response to hypoxia; response to UV; retinoic acid receptor signaling pathway; SMAD protein nuclear translocation; transforming growth factor beta receptor signaling pathway
Research Articles on PML
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Product Notes
The Mouse PML pml (Catalog #AAA9717328) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717328 ELISA Kit recognizes Mouse PML. It is sometimes possible for the material contained within the vial of "Protein PML (PML), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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