TAF1B blocking peptide

TAF1B Blocking Peptide

Cat. Num. AAA9622634

• Gene Names: TAF1B; SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: TAF1B; TAF1B Blocking Peptide; MGC:9349; RAF1B; RAFI63; SL1; TAB1 B; TAF1B TATA box binding protein (TBP) associated factor; RNA polymerase I; B; 63kDa; TAFI63; TATA box binding protein associated factor 1B; TATA box binding protein associated factor RNA polymerase I subunit B; TAF1B blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for TAF1B blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TAF1B blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 974987474
• NCBI GeneID: 9014
• NCBI Accession #: NP_001305905.1
• NCBI GenBank Nucleotide #: NM_001318976.1
• UniProt Accession #: Q53T94; Q15574; Q8WVC3; B4DI42; F8WD72
• Molecular Weight: 39,533 Da
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit B isoform b
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor, RNA polymerase I subunit B
• NCBI Official Symbol: TAF1B
• NCBI Official Synonym Symbols: SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit B
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF1B
• UniProt Synonym Gene Names: TAFI63
• UniProt Entry Name: TAF1B_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes one of the SL1-specific TAFs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

TAF1B: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3. Belongs to the RRN7/TAF1B family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex; DNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; TATA-binding protein binding; metal ion binding; transcription factor activity

Biological Process: chromatin silencing at rDNA; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic