SUB1 blocking peptide

SUB1 Blocking Peptide

Gene Names

SUB1; P15; PC4; p14

Applications

Blocking/Control

Purity

The purity is >90%,tested by HPLC and MS.

Synonyms

SUB1; SUB1 Blocking Peptide; Activated RNA polymerase II transcriptional coactivator p15; Interferon related developmental regulator 1; MGC102747; p14; P15; PC4; PC4 LSB; Positive cofactor 4; RPO2TC1; Sub1; SUB1 homolog; TCP4_HUMAN; SUB1 blocking peptide

Ordering
Cat. Num. AAA9621462 For Research Use Only!

Purity/Purification

The purity is >90%,tested by HPLC and MS.

Form/Format

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

Applicable Applications for SUB1 blocking peptide

Blocking/Control

Notes

For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)

Preparation and Storage

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SUB1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

NCBI GI #

1709514

NCBI GeneID

10923

UniProt Accession #

P53999; Q96L29

Molecular Weight

127

NCBI Official Full Name

Activated RNA polymerase II transcriptional coactivator p15

NCBI Official Synonym Full Names

SUB1 homolog (S. cerevisiae)

NCBI Official Symbol

SUB1

NCBI Official Synonym Symbols

P15; PC4; p14

NCBI Protein Information

activated RNA polymerase II transcriptional coactivator p15; positive cofactor 4; activated RNA polymerase II transcription cofactor 4

UniProt Protein Name

Activated RNA polymerase II transcriptional coactivator p15

Protein Family

Subtilisin

UniProt Gene Name

SUB1

UniProt Synonym Gene Names

PC4; RPO2TC1; PC4

UniProt Entry Name

TCP4_HUMAN

Uniprot Description

PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

Protein type: DNA-binding; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: transcription factor complex; nucleolus; nucleus

Molecular Function: protein binding; transcription coactivator activity; single-stranded DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

Research Articles on SUB1

1. p38 MAP kinase mediated HNF4 Ser158 phosphorylation (P-HNF4-S158), binding of PC4 to P-HNF4-S158 and characterize the functional domain of PC4 required for P-HNF4-S158 binding.

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Product Notes

The SUB1 sub1 (Catalog #AAA9621462) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SUB1 can be used in a range of immunoassay formats including, but not limited to, Blocking/Control. Researchers should empirically determine the suitability of the SUB1 sub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUB1, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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SUB1 blocking peptide