Rabbit anti-Human IL34 Polyclonal Antibody | anti-IL34 antibody

IL34, NT (IL34, C16orf77, Interleukin-34) (AP)

Cat. Num. AAA6310245

• Gene Names: IL34; IL-34; C16orf77
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IL34; Polyclonal Antibody; NT (IL34; C16orf77; Interleukin-34) (AP); Interleukin-34; anti-IL34 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-IL34 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: IL34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-53 amino acids from the N-terminal region of human IL34.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(IL34 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the IL34 antibody detected the IL34 protein (arrow).)

Related Product Information for anti-IL34 antibody

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).

Product Categories/Family for anti-IL34 antibody

Antibodies; Interleukin

References

Wei, S., et al. J. Leukoc. Biol. 88(3):495-505(2010). Lin, H., et al. Science 320(5877):807-811(2008). Lim, J., et al. Cell 125(4):801-814(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 289666750
• NCBI GeneID: 146433
• NCBI Accession #: NP_001166242.1
• NCBI GenBank Nucleotide #: NM_001172771.1
• UniProt Accession #: Q6ZMJ4; Q8N6L2; B2RC28; B2Z4A8; B2ZC70
• Molecular Weight: 27,354 Da
• NCBI Official Full Name: interleukin-34 isoform 2
• NCBI Official Synonym Full Names: interleukin 34
• NCBI Official Symbol: IL34
• NCBI Official Synonym Symbols: IL-34; C16orf77
• NCBI Protein Information: interleukin-34
• UniProt Protein Name: Interleukin-34
• Protein Family: Interleukin
• UniProt Gene Name: IL34
• UniProt Synonym Gene Names: C16orf77; IL-34
• UniProt Entry Name: IL34_HUMAN

NCBI Description

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

Uniprot Description

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Cytokine

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of cell proliferation; innate immune response; positive regulation of protein amino acid phosphorylation; inflammatory response