SMC1A blocking peptide

SMC1A Antibody (N-term) Blocking peptide

• Gene Names: SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• Synonyms: SMC1A; SMC1A Antibody (N-term) Blocking peptide; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb18; DXS423E; KIAA0178; SB18; SMC1; SMC1L1; SMC1A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of SMC1A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1233

• Cellular Location: Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note: Associates with chromatin. Before prophase it is scattered along chromosome arms During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere- kinetochore complex at the kinetochore region during mitosis
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SMC1A blocking peptide

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

NCBI and Uniprot Product Information

• NCBI GI #: 29336622
• NCBI GeneID: 8243
• UniProt Accession #: Q14683; O14995; Q16351; Q2M228
• Molecular Weight: 143,233 Da
• NCBI Official Full Name: Structural maintenance of chromosomes protein 1A
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 1A
• NCBI Official Symbol: SMC1A
• NCBI Official Synonym Symbols: SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• NCBI Protein Information: structural maintenance of chromosomes protein 1A
• UniProt Protein Name: Structural maintenance of chromosomes protein 1A
• Protein Family: Structural maintenance of chromosomes protein
• UniProt Gene Name: SMC1A
• UniProt Synonym Gene Names: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
• UniProt Entry Name: SMC1A_HUMAN

NCBI Description

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.

Protein type: RNA splicing; DNA replication

Chromosomal Location of Human Ortholog: Xp11.22-p11.21

Cellular Component: chromosome; chromosome, pericentric region; cohesin core heterodimer; condensed nuclear chromosome; cytoplasm; cytosol; kinetochore; nucleoplasm; nucleus

Molecular Function: chromatin binding; protein binding; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction; DNA repair; meiosis; mitotic cell cycle checkpoint; mitotic sister chromatid cohesion; mitotic sister chromatid segregation; mitotic spindle organization and biogenesis; negative regulation of DNA endoreduplication; protein sumoylation; response to radiation; sister chromatid cohesion

Disease: Cornelia De Lange Syndrome 2