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Western Blot (WB) (Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

SHISA3 blocking peptide

SHISA3 Peptide

Gene Names
SHISA3; hShisa3
Applications
Blocking (BL)
Synonyms
SHISA3; SHISA3 Peptide; hShisa3; Protein shisa-3 homolog; shisa homolog 3 (Xenopus laevis); SHISA3 blocking peptide
Ordering
For Research Use Only!
Form/Format
Liquid
Concentration
200 ug/mL (varies by lot)
Sequence Length
238
Applicable Applications for SHISA3 blocking peptide
Blocking (BL)
Application Notes
SHISA3 peptide is used for blocking the activity of SHISA3 antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 degree C.
Species
Human
Buffer
PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
Location
15 amino acids near the center of human SHISA3.
Preparation and Storage
Store SHISA3 peptide at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,832 Da
NCBI Official Full Name
protein shisa-3 homolog
NCBI Official Synonym Full Names
shisa family member 3
NCBI Official Symbol
SHISA3
NCBI Official Synonym Symbols
hShisa3
NCBI Protein Information
protein shisa-3 homolog; shisa homolog 3
UniProt Protein Name
Protein shisa-3 homolog
Protein Family
UniProt Gene Name
SHISA3
UniProt Entry Name
SHSA3_HUMAN

Uniprot Description

SHISA3: Plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. Belongs to the shisa family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p13

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Biological Process: multicellular organismal development

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Product Notes

The SHISA3 shisa3 (Catalog #AAA153996) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SHISA3 can be used in a range of immunoassay formats including, but not limited to, Blocking (BL). SHISA3 peptide is used for blocking the activity of SHISA3 antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 degree C. Researchers should empirically determine the suitability of the SHISA3 shisa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHISA3, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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