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Western Blot (WB) (Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

RWDD3 blocking peptide

RWDD3 Peptide

Gene Names
RWDD3; RSUME
Applications
Blocking (BL)
Synonyms
RWDD3; RWDD3 Peptide; RSUME; RWD domain-containing protein 3; RWD-containing sumoylation enhancer; RWD domain containing 3; RWDD3 blocking peptide
Ordering
For Research Use Only!
Form/Format
Liquid
Concentration
200 ug/mL (varies by lot)
Sequence Length
267
Applicable Applications for RWDD3 blocking peptide
Blocking (BL)
Application Notes
RWDD3 peptide is used for blocking the activity of RWDD3 antibody.
Species
Human
Buffer
PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
Location
15 amino acids near the carboxy terminus of human RWDD3.
Preparation and Storage
Store RWDD3 peptide at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,857 Da
NCBI Official Full Name
RWD domain-containing protein 3 isoform a
NCBI Official Synonym Full Names
RWD domain containing 3
NCBI Official Symbol
RWDD3
NCBI Official Synonym Symbols
RSUME
NCBI Protein Information
RWD domain-containing protein 3; RWD-containing sumoylation enhancer; RWD domain-containing sumoylation enhancer
UniProt Protein Name
RWD domain-containing protein 3
UniProt Gene Name
RWDD3
UniProt Synonym Gene Names
RSUME; RSUME
UniProt Entry Name
RWDD3_HUMAN

Uniprot Description

RWDD3: Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: SUMO conjugating system

Chromosomal Location of Human Ortholog: 1p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of protein sumoylation; inhibition of NF-kappaB transcription factor

Research Articles on RWDD3

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Product Notes

The RWDD3 rwdd3 (Catalog #AAA151978) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RWDD3 can be used in a range of immunoassay formats including, but not limited to, Blocking (BL). RWDD3 peptide is used for blocking the activity of RWDD3 antibody. Researchers should empirically determine the suitability of the RWDD3 rwdd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RWDD3, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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