Runx1 blocking peptide

Mouse Runx1 Blocking Peptide (C-term)

• Gene Names: Runx1; AML1; Cbfa2; Pebp2a2; Pebpa2b; CBF-alpha-2
• Synonyms: Runx1; Mouse Runx1 Blocking Peptide (C-term); Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; Aml1; Cbfa2; Pebp2ab; Runx1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 426-439 of HUMAN Runx1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 451

• Cellular Location: Nucleus.
• Tissue Location: Isoform 4 is expressed at high levels in thymus, spleen and T-cell lines and at lower levels in myeloid cell lines and nonhematopoietic cells. Isoform 5 is expressed ubiquitously in lumbar vertebrae, brain, kidney, heart, muscle, ovary and osteoblast-like cell line MC3T3-E1
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Runx1 blocking peptide

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B- dependent transcriptional activation (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 2498127
• NCBI GeneID: 12394
• UniProt Accession #: Q03347; O08598; Q62049; Q9ESB9; Q9ET65
• Molecular Weight: 33,485 Da
• NCBI Official Full Name: Runt-related transcription factor 1
• NCBI Official Synonym Full Names: runt related transcription factor 1
• NCBI Official Symbol: Runx1
• NCBI Official Synonym Symbols: AML1; Cbfa2; Pebp2a2; Pebpa2b; CBF-alpha-2
• NCBI Protein Information: runt-related transcription factor 1
• UniProt Protein Name: Runt-related transcription factor 1
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: Runx1
• UniProt Synonym Gene Names: Aml1; Cbfa2; Pebp2ab; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
• UniProt Entry Name: RUNX1_MOUSE

Uniprot Description

AML1: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and ALYREF/THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1. Interacts with YAP1. Interacts with HIPK2. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity. Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Cellular Component: basement membrane; cytoplasm; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: calcium ion binding; DNA binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity; transcription factor binding

Biological Process: behavioral response to pain; central nervous system development; chondrocyte differentiation; embryonic hemopoiesis; hair follicle morphogenesis; hemopoiesis; in utero embryonic development; liver development; myeloid cell differentiation; myeloid progenitor cell differentiation; negative regulation of cell proliferation; negative regulation of granulocyte differentiation; negative regulation of transcription, DNA-dependent; neuron development; neuron differentiation; neuron fate commitment; ossification; positive regulation of angiogenesis; positive regulation of granulocyte differentiation; positive regulation of interferon-gamma production; positive regulation of interleukin-2 production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of signal transduction; regulation of T cell anergy; regulation of transcription, DNA-dependent; response to retinoic acid; skeletal development