RPC6 blocking peptide

RPC6 Blocking Peptide

Cat. Num. AAA9628481

• Gene Names: POLR3F; RPC6; RPC39
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: RPC6; RPC6 Blocking Peptide; DNA directed RNA polymerase III 39 kDa polypeptide; DNA directed RNA polymerase III 39 kDa polypeptide F; DNA directed RNA polymerase III subunit F; DNA directed RNA polymerase III subunit RPC6; DNA directed RNA polymerases III 39 kDa polypeptide; DNA-directed RNA polymerase III subunit F; DNA-directed RNA polymerase III subunit RPC6; MGC13517; Polr3f; Polymerase (RNA) III (DNA directed) polypeptide F; RNA polymerase III 39 kDa subunit; RNA polymerase III C39 subunit; RNA polymerase III subunit C6; RPC 39; RPC 6; RPC39; RPC6_HUMAN; RPC6 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for RPC6 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RPC6 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 542133141
• NCBI GeneID: 10621
• NCBI Accession #: NP_001269455.1
• NCBI GenBank Nucleotide #: NM_001282526.1
• UniProt Accession #: Q9H1D9; O15319; A8K4C7
• Molecular Weight: 35,684 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC6 isoform 2
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
• NCBI Official Symbol: POLR3F
• NCBI Official Synonym Symbols: RPC6; RPC39
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III C39 subunit; RNA polymerase III subunit C6
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: POLR3F
• UniProt Synonym Gene Names: RNA polymerase III subunit C6; RPC39
• UniProt Entry Name: RPC6_HUMAN

NCBI Description

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Protein type: Transcription initiation complex; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter