Rabbit anti-Human, Mouse RPC6 Polyclonal Antibody | anti-RPC6 antibody

RPC6 Antibody

Cat. Num. AAA9609777

• Gene Names: POLR3F; RPC6; RPC39
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RPC6; Polyclonal Antibody; RPC6 Antibody; DNA directed RNA polymerase III 39 kDa polypeptide; DNA directed RNA polymerase III 39 kDa polypeptide F; DNA directed RNA polymerase III subunit F; DNA directed RNA polymerase III subunit RPC6; DNA directed RNA polymerases III 39 kDa polypeptide; DNA-directed RNA polymerase III subunit F; DNA-directed RNA polymerase III subunit RPC6; MGC13517; Polr3f; Polymerase (RNA) III (DNA directed) polypeptide F; RNA polymerase III 39 kDa subunit; RNA polymerase III C39 subunit; RNA polymerase III subunit C6; RPC 39; RPC 6; RPC39; RPC6_HUMAN; anti-RPC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPC6 antibody detects endogenous levels of total RPC6
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-RPC6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human RPC6
• Subcellular Location: Nucleus.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of RPC6 using HeLa whole cell lysates)

Related Product Information for anti-RPC6 antibody

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628).
Subunit Structure: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex (By similarity). Directly interacts with POLR3C (PubMed:21358628). Interacts with TBP and TFIIIB90 and GTF3C4 (By similarity) (PubMed:10523658).
Similarity: Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

NCBI and Uniprot Product Information

• NCBI GI #: 542133141
• NCBI GeneID: 10621
• NCBI Accession #: NP_001269455.1
• NCBI GenBank Nucleotide #: NM_001282526.1
• UniProt Accession #: Q9H1D9; O15319; A8K4C7
• Molecular Weight: Observed: 36 kDa; Predicted: 36 kDa
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC6 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase III subunit F
• NCBI Official Symbol: POLR3F
• NCBI Official Synonym Symbols: RPC6; RPC39
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC6
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: POLR3F
• UniProt Synonym Gene Names: RNA polymerase III subunit C6; RPC39

NCBI Description

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA) (PubMed:21358628).