RFWD2 blocking peptide

RFWD2 Blocking Peptide (C-term)

• Gene Names: RFWD2; COP1; RNF200
• Synonyms: RFWD2; RFWD2 Blocking Peptide (C-term); E3 ubiquitin-protein ligase RFWD2; 632-; Constitutive photomorphogenesis protein 1 homolog; hCOP1; RING finger and WD repeat domain protein 2; RING finger protein 200; COP1; RNF200; RFWD2 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 687-701 of HUMAN RFWD2
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 731

• Cellular Location: Nucleus speckle. Cytoplasm. Note: In the nucleus, it forms nuclear speckles
• Tissue Location: Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RFWD2 blocking peptide

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 21359963
• NCBI GeneID: 64326
• NCBI Accession #: NP_071902.2
• NCBI GenBank Nucleotide #: NM_022457.6
• UniProt Accession #: Q8NHY2; Q504W6; Q6H103; Q9H6L7; E9PKI0; X5D9B4
• Molecular Weight: 14,914 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RFWD2 isoform a
• NCBI Official Synonym Full Names: ring finger and WD repeat domain 2
• NCBI Official Symbol: RFWD2
• NCBI Official Synonym Symbols: COP1; RNF200
• NCBI Protein Information: E3 ubiquitin-protein ligase RFWD2
• UniProt Protein Name: E3 ubiquitin-protein ligase RFWD2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RFWD2
• UniProt Synonym Gene Names: COP1; RNF200; hCOP1
• UniProt Entry Name: RFWD2_HUMAN

Uniprot Description

COP1: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Homodimer. Homodimerization is mediated by the coiled coil domain. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Isoform 2 does not interact with CUL4A but still binds to RBX1, suggesting that the interaction may be mediated by another culllin protein. Isoform 1 and isoform 2 interact with CUL5 but not with CUL1, CUL2 not CUL3. Interacts with bZIP transcription factors JUN, JUNB and JUND but not with FOS, ATF2 nor XBP1. Interacts with p53 (TP53). By p53/TP53. Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. Belongs to the COP1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 1q25.1-q25.2

Cellular Component: centrosome; cytoplasm; cytosol; focal adhesion; nucleoplasm

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; response to ionizing radiation