RASGRP2 blocking peptide

RASGRP2 Blocking Peptide (N-term)

• Gene Names: RASGRP2; CDC25L; CALDAG-GEFI
• Synonyms: RASGRP2; RASGRP2 Blocking Peptide (N-term); RAS guanyl-releasing protein 2; Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B33 kinase-like protein; CDC25L; MCG7; RASGRP2 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 128-142 of HUMAN RASGRP2
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 609

• Cellular Location: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note: Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity)
• Tissue Location: Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RASGRP2 blocking peptide

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 74713056
• NCBI GeneID: 10235
• UniProt Accession #: Q7LDG7; O00538; Q9UL65; A6NDC7
• Molecular Weight: 69,320 Da
• NCBI Official Full Name: RAS guanyl-releasing protein 2
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 2
• NCBI Official Symbol: RASGRP2
• NCBI Official Synonym Symbols: CDC25L; CALDAG-GEFI
• NCBI Protein Information: RAS guanyl-releasing protein 2
• UniProt Protein Name: RAS guanyl-releasing protein 2
• Protein Family: RAS guanyl-releasing protein
• UniProt Gene Name: RASGRP2
• UniProt Synonym Gene Names: CDC25L; MCG7; CalDAG-GEFI; hCDC25L
• UniProt Entry Name: GRP2_HUMAN

NCBI Description

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.