RAPGEF2 blocking peptide

RAPGEF2 Blocking Peptide (C-term)

• Gene Names: RAPGEF2; RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
• Synonyms: RAPGEF2; RAPGEF2 Blocking Peptide (C-term); Rap guanine nucleotide exchange factor 2; Cyclic nucleotide ras GEF; CNrasGEF; Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA-GEF-1; Ras/Rap1-associating GEF-1; KIAA0313; NRAPGEP; PDZGEF1; RAPGEF2 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 1464-1478 of HUMAN RAPGEF2
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1499

• Cellular Location: Cytoplasm. Cytoplasm, perinuclear region. Cell membrane. Late endosome. Cell junction Note: Associated with the synaptic plasma membrane. Colocalizes with ADRB1 at the plasma membrane. Synaptosome. Enriched in synaptic plasma membrane and neuronal cell body. Colocalized with CTNNB1 at cell-cell contacts (By similarity). Localized diffusely in the cytoplasm before neuronal growth factor (NGF) stimulation Recruited to late endosomes after NGF stimulation. Colocalized with the high affinity nerve growth factor receptor NTRK1 at late endosomes. Translocated to the perinuclear region in a RAP1A- dependent manner. Translocated to the cell membrane
• Tissue Location: Expressed in primary neuronal and endocrine cells (at protein level). Highest expression levels in brain Lower expression levels in heart, kidney, lung, placenta and blood leukocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RAPGEF2 blocking peptide

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:23800469, PubMed:10801446) or not (PubMed:10608844, PubMed:10548487, PubMed:11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP- induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain- derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

NCBI and Uniprot Product Information

• NCBI GI #: 34395737
• NCBI GeneID: 9693
• UniProt Accession #: Q9Y4G8; D3DP27
• Molecular Weight: 167,417 Da
• NCBI Official Full Name: Rap guanine nucleotide exchange factor 2
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor 2
• NCBI Official Symbol: RAPGEF2
• NCBI Official Synonym Symbols: RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
• NCBI Protein Information: rap guanine nucleotide exchange factor 2
• UniProt Protein Name: Rap guanine nucleotide exchange factor 2
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF2
• UniProt Synonym Gene Names: KIAA0313; NRAPGEP; PDZGEF1; CNrasGEF; nRap GEP; PDZ-GEF1
• UniProt Entry Name: RPGF2_HUMAN

NCBI Description

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAPGEF2: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP. Interacts with MAGI2. Highest expression levels in brain. Lower expression levels in heart, kidney, lung, and placenta.

Protein type: GEFs, Ras; Motility/polarity/chemotaxis; GEFs

Chromosomal Location of Human Ortholog: 4q32.1

Cellular Component: apical plasma membrane; cell soma; cytoplasm; cytosol; endocytic vesicle; intercellular junction; late endosome; membrane; neuron projection; perinuclear region of cytoplasm; plasma membrane; protein complex; synapse; tight junction

Molecular Function: beta-1 adrenergic receptor binding; cAMP binding; cGMP binding; GTPase activator activity; PDZ domain binding; protein binding; Rap guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity; signal transducer activity; WW domain binding

Biological Process: blood vessel development; brain-derived neurotrophic factor receptor signaling pathway; cAMP-mediated signaling; forebrain neuron development; G-protein coupled receptor protein signaling pathway; MAPKKK cascade; microvillus biogenesis; negative regulation of cell proliferation; negative regulation of dendrite morphogenesis; negative regulation of melanin biosynthetic process; neurite development; neuron migration; neuropeptide signaling pathway; positive regulation of GTPase activity; positive regulation of protein binding; positive regulation of protein kinase activity; Rap protein signal transduction; regulation of synaptic plasticity; small GTPase mediated signal transduction; ventricular system development