Typical Testing Data/Standard Curve (for reference only)
Mouse RAPGEF2 ELISA Kit | RAPGEF2 elisa kit
Mouse RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RAPGEF2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RAPGEF2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RAPGEF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of RAPGEF2. You can calculate the concentration of RAPGEF2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
Uniprot Description
RAPGEF2: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP. Interacts with MAGI2. Highest expression levels in brain. Lower expression levels in heart, kidney, lung, and placenta.
Protein type: GEFs, Ras; GEFs; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q32.1
Cellular Component: neuron projection; tight junction; protein complex; integral to plasma membrane; intercellular junction; cytosol; cell soma; membrane; endocytic vesicle; perinuclear region of cytoplasm; late endosome; apical plasma membrane; cytoplasm; plasma membrane; synapse
Molecular Function: diacylglycerol binding; signal transducer activity; protein binding; Rap guanyl-nucleotide exchange factor activity; beta-1 adrenergic receptor binding; WW domain binding; cGMP binding; calcium ion binding; cAMP binding; protein kinase binding; Ras guanyl-nucleotide exchange factor activity; PDZ domain binding
Biological Process: blood vessel development; positive regulation of protein binding; brain-derived neurotrophic factor receptor signaling pathway; ventricular system development; neuron migration; MAPKKK cascade; regulation of synaptic plasticity; negative regulation of dendrite morphogenesis; microvillus biogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cAMP-mediated signaling; forebrain neuron development; neuropeptide signaling pathway; small GTPase mediated signal transduction; positive regulation of protein kinase activity; negative regulation of melanin biosynthetic process; Rap protein signal transduction; neurite development
Research Articles on RAPGEF2
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Product Notes
The Mouse RAPGEF2 rapgef2 (Catalog #AAA2514890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2514890 ELISA Kit recognizes Mouse RAPGEF2. It is sometimes possible for the material contained within the vial of "RAPGEF2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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