RAB21 blocking peptide

RAB21 Antibody (C-term) Blocking peptide

• Synonyms: RAB21; RAB21 Antibody (C-term) Blocking peptide; Ras-related protein Rab-21; KIAA0118; RAB21 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of RAB21. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 225

• Cellular Location: Endoplasmic reticulum membrane; Lipid-anchor. Golgi apparatus, trans- Golgi network. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane Cleavage furrow. Note: Colocalizes with ANKRD27 and VAMP7 in neurites (By similarity). In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid- telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
• Tissue Location: Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RAB21 blocking peptide

Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Involved in neurite growth (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 13633613
• NCBI GeneID: 23011
• UniProt Accession #: Q9UL25; Q14466; Q569H3
• Molecular Weight: 24,348 Da
• NCBI Official Full Name: Ras-related protein Rab-21
• NCBI Official Synonym Full Names: RAB21, member RAS oncogene family
• NCBI Official Symbol: RAB21
• NCBI Protein Information: ras-related protein Rab-21
• UniProt Protein Name: Ras-related protein Rab-21
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB21
• UniProt Synonym Gene Names: KIAA0118
• UniProt Entry Name: RAB21_HUMAN

NCBI Description

This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015]

Uniprot Description

RAB21: Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Belongs to the small GTPase superfamily. Rab family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 12q21.1

Cellular Component: endosome; focal adhesion; internal side of plasma membrane; trans-Golgi network; vesicle membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: positive regulation of receptor-mediated endocytosis; regulation of exocytosis