Mouse RAB21 Monoclonal Antibody | anti-RAB21 antibody

RAB21 Antibody

Cat. Num. AAA9217065

• Reactivity: Human, African Green Monkey
• Applications: Western Blot, ELISA
• Synonyms: RAB21; Monoclonal Antibody; RAB21 Antibody; Ras-related protein Rab-21; KIAA0118; anti-RAB21 antibody

• Host: Mouse
• Reactivity: Human, African Green Monkey
• Clonality: Monoclonal
• Isotype: IgG2b,kappa
• Specificity: This RAB21 antibody is generated from a mouse immunized with a recombinant protein between 1-225 amino acids from the human RAB21.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 225

• Applicable Applications for anti-RAB21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:2000
• Subtitle: Purified Mouse Monoclonal Antibody (Mab)
• Cellular Location: Endoplasmic reticulum membrane; Lipid-anchor. Golgi apparatus, trans- Golgi network. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane Cleavage furrow. Note=Colocalizes with ANKRD27 and VAMP7 in neurites (By similarity). In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid- telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells. {ECO:0000250|UniProtKB:P35282, ECO:0000250|UniProtKB:Q6AXT5}.
• Tissue Location: Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RAB21 Antibody at 1:2000 dilutionLane 1: MCF-7 whole cell lysateLane 2: A431 whole cell lysateLane 3: Hela whole cell lysateLane 4: COS-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RAB21 antibody

Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Involved in neurite growth (By similarity).

Product Categories/Family for anti-RAB21 antibody

Crown Antibodies; Primary Antibodies; Cancer; Cell Biology; Signal Transduction

References

Opdam F.J.M.,et al.Eur. J. Cell Biol. 79:308-316 (2000). Bienvenut W.V.,et al.Submitted (JUN-2005) to UniProtKB. Nagase T.,et al.DNA Res. 2:37-43 (1995). Simpson J.C.,et al.J. Cell Sci. 117:6297-6311 (2004). Zhang X.,et al.J. Cell Sci. 119:1053-1062 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 7661922
• NCBI GeneID: 23011
• NCBI Accession #: NP_055814.1
• NCBI GenBank Nucleotide #: NM_014999.3
• UniProt Accession #: Q9UL25; Q14466; Q569H3
• Molecular Weight: 24348 Da
• NCBI Official Full Name: ras-related protein Rab-21
• NCBI Official Synonym Full Names: RAB21, member RAS oncogene family
• NCBI Official Symbol: RAB21
• NCBI Protein Information: ras-related protein Rab-21
• UniProt Protein Name: Ras-related protein Rab-21
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB21
• UniProt Synonym Gene Names: KIAA0118
• UniProt Entry Name: RAB21_HUMAN

NCBI Description

This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015]

Uniprot Description

RAB21: Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration. During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein, monomeric; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q21.1

Cellular Component: cleavage furrow; cytoplasmic vesicle membrane; endoplasmic reticulum membrane; endosome; focal adhesion; internal side of plasma membrane; trans-Golgi network; vesicle membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: anterograde axon cargo transport; intracellular protein transport; metabolic process; nucleocytoplasmic transport; positive regulation of dendrite morphogenesis; positive regulation of receptor-mediated endocytosis; regulation of axon extension; regulation of exocytosis; small GTPase mediated signal transduction