PTF1A blocking peptide

PTF1A Antibody (C-term) Blocking peptide

• Gene Names: PTF1A; PACA; PAGEN2; bHLHa29; PTF1-p48
• Synonyms: PTF1A; PTF1A Antibody (C-term) Blocking peptide; Pancreas transcription factor 1 subunit alpha; Class A basic helix-loop-helix protein 29; bHLHa29; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1-p48; BHLHA29; PTF1P48; PTF1A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of PTF1A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 328

• Cellular Location: Nucleus. Cytoplasm. Note: In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex (By similarity).
• Tissue Location: Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PTF1A blocking peptide

Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.

NCBI and Uniprot Product Information

• NCBI GI #: 74749931
• NCBI GeneID: 256297
• UniProt Accession #: Q7RTS3; Q9HC25
• Molecular Weight: 34,970 Da
• NCBI Official Full Name: Pancreas transcription factor 1 subunit alpha
• NCBI Official Synonym Full Names: pancreas specific transcription factor, 1a
• NCBI Official Symbol: PTF1A
• NCBI Official Synonym Symbols: PACA; PAGEN2; bHLHa29; PTF1-p48
• NCBI Protein Information: pancreas transcription factor 1 subunit alpha
• UniProt Protein Name: Pancreas transcription factor 1 subunit alpha
• Protein Family: Pancreas transcription factor
• UniProt Gene Name: PTF1A
• UniProt Synonym Gene Names: BHLHA29; PTF1P48; bHLHa29; PTF1-p48
• UniProt Entry Name: PTF1A_HUMAN

NCBI Description

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: cytoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding

Biological Process: cerebellum development; exocrine pancreas development; pancreas development; regulation of transcription, DNA-dependent; tissue development

Disease: Pancreatic Agenesis 2; Pancreatic And Cerebellar Agenesis