PRAS40 blocking peptide

PRAS40 Blocking Peptide

Cat. Num. AAA9615346

• Gene Names: AKT1S1; Lobe; PRAS40
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: PRAS40; PRAS40 Blocking Peptide; 40kDa proline rich AKT substrate; 40kDa proline-rich AKT substrate; AKT1 S1; AKT1 substrate 1 (proline rich); AKT1 substrate 1; AKT1S 1; AKT1S1; AKTS1_HUMAN; Lobe; MGC2865; PRAS 40; PRAS; Proline rich akt substrate; Proline rich Akt substrate 40kDa; Proline-rich AKT1 substrate 1; PRAS40 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for PRAS40 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PRAS40 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 148806906
• NCBI GeneID: 84335
• NCBI Accession #: NP_001092102.1
• NCBI GenBank Nucleotide #: NM_001098632.2
• UniProt Accession #: Q96B36; Q96BI4; Q96IK7; Q96NG2; Q9BWR5; A8MTQ1; B2RE93
• Molecular Weight: 27,383 Da
• NCBI Official Full Name: proline-rich AKT1 substrate 1 isoform b
• NCBI Official Synonym Full Names: AKT1 substrate 1 (proline-rich)
• NCBI Official Symbol: AKT1S1
• NCBI Official Synonym Symbols: Lobe; PRAS40
• NCBI Protein Information: proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
• UniProt Protein Name: Proline-rich AKT1 substrate 1
• UniProt Gene Name: AKT1S1
• UniProt Synonym Gene Names: PRAS40
• UniProt Entry Name: AKTS1_HUMAN

NCBI Description

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. Ref.6 Ref.8 Ref.9

Subunit structure: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins. Ref.5 Ref.8 Ref.9

Subcellular location: Cytoplasm › cytosol

By similarity. Note: Found in the cytosolic fraction of the brain

By similarity.

Tissue specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). Ref.5 Ref.6

Post-translational modification: Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1. Ref.5

Sequence caution: The sequence AAH00031.2 differs from that shown. Reason: Erroneous initiation.