Western Blot (WB)
(Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.)
Rabbit HIPK3 Polyclonal Antibody | anti-HIPK3 antibody
HIPK3 Antibody (C-term)
Western Blot (WB)
(Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.)
Western Blot (WB)
(Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.
NCBI and Uniprot Product Information
Uniprot Description
HIPK3: homeodomain interacting protein kinase 3 is a CMGC kinase of the DYRK family. Phosphorylates FADD and inhibits fas-mediated Jun NH(2)-terminal kinase activation.
Protein type: Nuclear receptor co-regulator; Kinase, protein; Protein kinase, CMGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CMGC group; DYRK family; HIPK subfamily
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: PML body; cytoplasm; nucleus
Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity
Biological Process: negative regulation of JNK activity; peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; apoptosis; peptidyl-threonine phosphorylation; mRNA transcription; protein amino acid phosphorylation; negative regulation of apoptosis
Research Articles on HIPK3
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Product Notes
The HIPK3 hipk3 (Catalog #AAA9206864) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1127-1156. The HIPK3 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's HIPK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the HIPK3 hipk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIPK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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