PPP2R2A blocking peptide

PPP2R2A Blocking Peptide

Cat. Num. AAA9626277

• Gene Names: PPP2R2A; B55A; PR52A; PR55A; B55ALPHA; PR55alpha
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: PPP2R2A; PPP2R2A Blocking Peptide; 2ABA_HUMAN; Alpha isoform of regulatory subunit B55 protein phosphatase 2; B55A; B55ALPHA; calcineurin; PP2A subunit B B alpha isoform; PP2A subunit B B55 alpha isoform; PP2A subunit B isoform alpha; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B PR55 alpha isoform; PP2A subunit B R2 alpha isoform; PR52A; PR55A; Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) alpha isoform; Protein phosphatase 2 (formerly 2A); regulatory subunit B (PR52); alpha isoform; Protein phosphatase 2 regulatory subunit B alpha; Protein phosphatase 2 regulatory subunit B alpha isoform; Protein phosphatase 2 regulatory subunit Balpha; Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; Testicular tissue protein Li 156; PPP2R2A blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for PPP2R2A blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PPP2R2A blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 294832006
• NCBI GeneID: 5520
• NCBI Accession #: NP_001171062.1
• NCBI GenBank Nucleotide #: NM_001177591.1
• UniProt Accession #: P63151; P50409; Q00007; B2RBU8; B4E1T7
• Molecular Weight: 53,000 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit Balpha
• NCBI Official Symbol: PPP2R2A
• NCBI Official Synonym Symbols: B55A; PR52A; PR55A; B55ALPHA; PR55alpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2R2A
• UniProt Entry Name: 2ABA_HUMAN

NCBI Description

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Uniprot Description

PPP2R2A: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Expressed in all tissues examined. Belongs to the phosphatase 2A regulatory subunit B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytosol

Molecular Function: protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; regulation of catalytic activity; mRNA catabolic process, nonsense-mediated decay; response to morphine; gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle; signal transduction; protein amino acid dephosphorylation