PIP5K1A blocking peptide

PIP5K1A Antibody (N-term) Blocking Peptide

• Synonyms: PIP5K1A; PIP5K1A Antibody (N-term) Blocking Peptide; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; PIP5KIalpha; PIP5K1A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human PIP5K1A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 562

• Cellular Location: Cell membrane. Cytoplasm. Nucleus speckle. Cell projection, ruffle. Note: Colocalizes with RAC1 at actin-rich membrane ruffles. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs
• Tissue Location: Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PIP5K1A blocking peptide

Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 74752158
• NCBI GeneID: 8394
• UniProt Accession #: Q99755; Q99754; Q99756; A8K4Q0; B4DIN0
• Molecular Weight: 58,119 Da
• NCBI Official Full Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
• NCBI Official Symbol: PIP5K1A
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase
• UniProt Gene Name: PIP5K1A
• UniProt Synonym Gene Names: PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha
• UniProt Entry Name: PI51A_HUMAN

Uniprot Description

PIP5K1A: Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Interacts with RAC1. Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation. Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; EC 2.7.1.68; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasm; cytosol; focal adhesion; lamellipodium; mRNA cleavage and polyadenylation specificity factor complex; nuclear speck; nucleoplasm; nucleus; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; kinase binding; protein binding

Biological Process: actin cytoskeleton reorganization; focal adhesion formation; glycerophospholipid metabolic process; keratinocyte differentiation; phagocytosis; phosphatidylinositol biosynthetic process; phospholipid biosynthetic process; regulation of phosphoinositide 3-kinase cascade; signal transduction