Pdk2 blocking peptide

Mouse Pdk2 Antibody (N-term) Blocking peptide

• Synonyms: Pdk2; Mouse Pdk2 Antibody (N-term) Blocking peptide; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; Pdk2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of Mouse Pdk2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 407

• Cellular Location: Mitochondrion matrix
• Tissue Location: Detected in heart (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Pdk2 blocking peptide

Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 341941241
• NCBI GeneID: 18604
• UniProt Accession #: Q9JK42; Q8VC63
• Molecular Weight: 46,041 Da
• NCBI Official Full Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isoenzyme 2
• NCBI Official Symbol: Pdk2
• NCBI Protein Information: pyruvate dehydrogenase kinase, isoenzyme 2
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• UniProt Gene Name: Pdk2
• UniProt Synonym Gene Names: PDH kinase 2
• UniProt Entry Name: PDK2_MOUSE

Uniprot Description

PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Mitochondrial; EC 2.7.11.2; Kinase, protein; Protein kinase, atypical; ATYPICAL group; PDHK family; PDHK subfamily

Cellular Component: cytoplasm; intracellular; mitochondrial pyruvate dehydrogenase complex; mitochondrion; nucleoplasm; pyruvate dehydrogenase complex

Molecular Function: ATP binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein serine/threonine kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity

Biological Process: cellular response to nutrient; glucose homeostasis; insulin receptor signaling pathway; peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of gluconeogenesis; regulation of pH