Pdgfra blocking peptide

Mouse Pdgfra Antibody (Center) Blocking Peptide

• Gene Names: Pdgfra; CD140a; Pdgfr-2; AI115593
• Synonyms: Pdgfra; Mouse Pdgfra Antibody (Center) Blocking Peptide; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; Platelet-derived growth factor alpha receptor; CD140a; Pdgfra blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1089

• Cellular Location: Cell membrane; Single-pass type I membrane protein. Note: The activated receptor is rapidly internalized and degraded.
• Tissue Location: Focally expressed in cortical interstitial cells and highly expressed in the interstitium of the papillary region. Also expressed by adventitial cells in arterial vessels Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, expression in cortical interstitial cells becomes prominent at day 4 which increases progressively until day 14.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Pdgfra blocking peptide

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 182705268
• NCBI GeneID: 18595
• UniProt Accession #: P26618; Q3TQ37; Q62046; Q7TSJ3; Q8C4N3
• Molecular Weight: 88,629 Da
• NCBI Official Full Name: Platelet-derived growth factor receptor alpha
• NCBI Official Synonym Full Names: platelet derived growth factor receptor, alpha polypeptide
• NCBI Official Symbol: Pdgfra
• NCBI Official Synonym Symbols: CD140a; Pdgfr-2; AI115593
• NCBI Protein Information: platelet-derived growth factor receptor alpha
• UniProt Protein Name: Platelet-derived growth factor receptor alpha
• Protein Family: Platelet-derived growth factor receptor
• UniProt Gene Name: Pdgfra
• UniProt Synonym Gene Names: PDGF-R-alpha; PDGFR-alpha
• UniProt Entry Name: PGFRA_MOUSE

Uniprot Description

PDGFRA: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation. Particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. Chromosomal rearrangments activate PDGFRalpha by fusion to BCR, causing atypical chronic myelogenous leukemia (CML), and to FIP1L1, causing idiopathic hypereosinophilic syndrome. Activating point mutations cause a minority of gastrointestinal stromal tumors (GIST). Promoter polymorphisms linked to neural tube defects including spina bifida, verified by mouse mutant model. Inhibitors: Gleevec, Sutent. OMIM: Two alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Oncoprotein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Cellular Component: axon; cell surface; cytoplasm; external side of plasma membrane; integral to plasma membrane; intrinsic to plasma membrane; membrane; microvillus; nucleus; plasma membrane; protein complex

Molecular Function: phosphoinositide 3-kinase binding; platelet-derived growth factor alpha-receptor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein complex binding; protein homodimerization activity; protein kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: adrenal gland development; anatomical structure morphogenesis; cardiac myofibril assembly; cell migration; elevation of cytosolic calcium ion concentration; embryonic cranial skeleton morphogenesis; embryonic digestive tract morphogenesis; estrogen metabolic process; extracellular matrix organization and biogenesis; female gonad development; hemopoietic progenitor cell differentiation; in utero embryonic development; Leydig cell differentiation; lung development; luteinization; male genitalia development; odontogenesis of dentine-containing teeth; organ morphogenesis; palate development; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; protein amino acid autophosphorylation; regulation of chemotaxis; skeletal morphogenesis; wound healing