PARL blocking peptide

PARL Blocking Peptide

Cat. Num. AAA9620242

• Gene Names: PARL; PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: PARL; PARL Blocking Peptide; Mitochondrial intramembrane cleaving protease PARL; Mitochondrial intramembrane-cleaving protease PARL; P-beta; parl; PARL_HUMAN; Pbeta; presenilin associated rhomboid-like; Presenilins associated rhomboid-like protein; PRO2207; PSARL; PSARL1; PSENIP2; RHBDS1; Rhomboid 7 homolog 1; PARL blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for PARL blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PARL blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 83641878
• NCBI GeneID: 55486
• NCBI Accession #: NP_001032728.1
• NCBI GenBank Nucleotide #: NM_001037639.2
• UniProt Accession #: Q9H300; Q96CQ4; Q9BTJ6; Q9P1E3
• Molecular Weight: 36,589 Da
• NCBI Official Full Name: presenilins-associated rhomboid-like protein, mitochondrial isoform 2
• NCBI Official Synonym Full Names: presenilin associated rhomboid like
• NCBI Official Symbol: PARL
• NCBI Official Synonym Symbols: PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
• NCBI Protein Information: presenilins-associated rhomboid-like protein, mitochondrial
• UniProt Protein Name: Presenilins-associated rhomboid-like protein, mitochondrial
• Protein Family: Presenilins-associated rhomboid-like protein
• UniProt Gene Name: PARL
• UniProt Synonym Gene Names: PSARL; Pbeta
• UniProt Entry Name: PARL_HUMAN

NCBI Description

This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease. [provided by RefSeq, May 2016]

Uniprot Description

PSARL: a mitochondrial intramembrane protease that is associated with insulin resistance and type 2 diabetes. Induced in humans by caloric restriction. Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. Induced in humans by caloric restriction. Interacts with PSEN1 and PSEN2. Binds OPA1. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral; Protease; EC 3.4.21.105; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: protein binding; serine-type endopeptidase activity; endopeptidase activity

Biological Process: membrane protein proteolysis; proteolysis