PAPSS2 blocking peptide

PAPSS2 Antibody (C-term) Blocking Peptide

• Gene Names: PAPSS2; SK2; BCYM4; ATPSK2
• Synonyms: PAPSS2; PAPSS2 Antibody (C-term) Blocking Peptide; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2; PAPS synthase 2; PAPSS 2; Sulfurylase kinase 2; SK 2; SK2; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase; ATPSK2; PAPSS2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human PAPSS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 619

• Tissue Location: Expressed in cartilage and adrenal gland.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PAPSS2 blocking peptide

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 62912492
• NCBI GeneID: 9060
• NCBI Accession #: NP_001015880
• NCBI GenBank Nucleotide #: NM_001015880.1
• UniProt Accession #: O95340; Q9BZL2; Q9P0G6; Q9UHM1; Q9UKD3; Q9UP30
• Molecular Weight: 69,970 Da
• NCBI Official Full Name: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 isoform b
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• NCBI Official Symbol: PAPSS2
• NCBI Official Synonym Symbols: SK2; BCYM4; ATPSK2
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• UniProt Protein Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS2
• UniProt Synonym Gene Names: ATPSK2; PAPS synthase 2; PAPSS 2; SK 2; SK2; SAT
• UniProt Entry Name: PAPS2_HUMAN

NCBI Description

Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PAPSS2: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. May have a important role in skeletogenesis during postnatal growth. Defects in PAPSS2 are the cause of spondyloepimetaphyseal dysplasia Pakistani type (SEMD-PA). A bone disease characterized by epiphyseal dysplasia with mild metaphyseal abnormalities. Clinical features include short stature evidenced at birth, short and bowed lower limbs, mild brachydactyly, kyphoscoliosis, abnormal gait, enlarged knee joints. Some patients may manifest premature pubarche and hyperandrogenism associated with skeletal dysplasia and short stature. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Transferase; Other Amino Acids Metabolism - selenoamino acid; EC 2.7.7.4; Energy Metabolism - sulfur; EC 2.7.1.25

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cytosol

Molecular Function: adenylylsulfate kinase activity; nucleotidyltransferase activity; sulfate adenylyltransferase (ATP) activity

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; skeletal development; sulfate assimilation

Disease: Brachyolmia Type 4 With Mild Epiphyseal And Metaphyseal Changes